FEI Tecnai F20 Transmission Electron Microscope for Core Microscopy Facility

描述(由申请人提供)：约翰霍普金斯大学艺术与科学学院、怀廷工程学院和JHMI医学院的9名主要用户和8名少数用户是资金充足的NIH调查人员，他们申请资金购买FEI Tecnai F20双200千伏传输电子显微镜(TEM)，带有Falcon II直接探测相机。这一新的最先进的透射电子显微镜将安置在霍普金斯大学霍姆伍德校区唯一的透射电子显微镜设施--霍普金斯综合成像中心(www.jhu.edu/iic)；霍普金斯大学的整个研究社区都很好地利用了这一设施。它将取代已有30年历史的飞利浦EM 420，其服务和日常可靠性已变得非常可疑(参见附录A中FEI东区服务经理John Fike的信函)；并对现有的Tecnai 12进行完美补充。如果获得资金，Tecnai F20将成为霍普金斯大学冷冻电子显微镜和低剂量应用的主要透射电子显微镜平台。其200千伏容量的FEG和Falcon II直接探测相机将在低温和分子透射电子显微镜中添加升级后的功能，令人惊讶的是，这些功能目前在霍普金斯大学的任何地方都不存在；它还将腾出我们现有的T12，用于更传统、常规的透射电子显微镜应用。我们的研究人员都是由NIH资助的，他们致力于一系列与细胞生物学、生化和生物分子相关的基本问题，包括但不限于：HIV结构和功能、HSV组装和成熟、DNA调节和染色体超结构、膜组织和动力学、内小体/溶酶体动力学、膜运输、聚合基因载体的运输、唾液酸展示和细胞黏附，以及内吞作用的机制。新的Tecnai F20将确保我们多样化的研究人员能够连续、不间断地访问针对许多不同应用而优化的TEM；并允许未来在更奇异的结构应用中发展CryoTEM和单粒子分析。Tecnai F20将在生物系即将翻新的综合成像中心(IIC)中方便地定位、管理和维护，这是霍姆伍德校园/霍普金斯大学范围内的一个显微镜资源，由&gt；150个实验室和&gt；400个用户组成的多个学校和部门定期使用。IIC是霍普金斯大学霍姆伍德校区的核心显微镜资源，与纳米生物技术研究所(INBT)和新成立的肿瘤学工程中心有关。它由克里格艺术与科学学院和怀廷工程学院共同支持；并由多所学校/系定期使用。新系统将被纳入IIC现有的充值系统，以确保收回用品和维护资金；它将通过我们方便的IIC网络调度器和智能手机应用程序免费提供给霍普金斯大学的所有感兴趣的用户。

DESCRIPTION (provided by applicant): A diverse group of 9 major and 8 minor users who are well funded NIH investigators, within the Johns Hopkins School of Arts & Sciences, Whiting School of Engineering, and the JHMI School of Medicine, request funds to purchase an FEI Tecnai F20 TWIN 200kV transmission electron microscope (TEM) with a Falcon II direct detection camera. This new state-of-the-art TEM will be housed in the Hopkins Integrated Imaging Center (www.jhu.edu/IIC) that is the only TEM facility on the Hopkins' Homewood campus; and is well utilized by the Hopkins-wide research community. It will replace a 30 year old Philips EM 420 for which service and day-to-day reliability has become critically dubious (see letter from John Fike, East Regional Service Manager, FEI in Appendix A); and perfectly complement an existing Tecnai 12. If funded, the Tecnai F20 will become the primary TEM platform for cryoelectron microscopy and low dose applications at Hopkins. Its 200kV capability, FEG, and Falcon II direct detection camera will add upgraded capabilities in cryo and molecular TEM, that are not currently present, astonishingly, anywhere at Hopkins; and it will free up our existing T12 for use in more conventional, routine TEM applications. Our investigators, all well-funded through the NIH, work on a host of basic cell biological, biochemical, and biomolecular related questions including, though not limited to: HIV structure and function, HSV assembly and maturation; DNA regulation and chromosome suprastructure, membrane organization and dynamics; endosome/lysosome dynamics; membrane trafficking; trafficking of polymeric gene carriers; sialic acid display and cell adhesion; and mechanisms of endocytosis. The new Tecnai F20 will ensure that our diverse group of investigators have continued and uninterrupted access to a TEM optimized for many disparate applications; as well as allowing for future growth in more exotic structural applications of cryoTEM and single particle analysis. The Tecnai F20 will be conveniently located, administered, and maintained, in the Biology Department's soon to be renovated Integrated Imaging Center (IIC), a Homewood campus/Hopkins-wide microscopy resource that is utilized regularly by multiple schools and departments comprising >150 laboratories and >400 users. The IIC is a Hopkins' Homewood campus core microscopy resource associated with the Institute for NanoBioTechnology (INBT); and the newly established Engineering in Oncology Center. It is jointly supported by the Krieger School of Arts and Sciences and the Whiting School of Engineering; and is utilized regularly by multiple schools/departments The new system will be incorporated into the IIC's existing recharge system to ensure recovery of funds for supplies and maintenance; and it will be made freely available to all interested users Hopkins-wide through our convenient IIC web-scheduler and smart phone app.