Animal care: supporting research on autoimmune, inflammatory and muscle diseases

LACU协助NIAMS IRP研究人员进行涉及动物的研究。这些动物饲养在NIH园区的共享动物设施中，包括位于10号楼9/C127室的NIAMS LACU，以及位于6 B、10A、50号楼和14号楼的门诊护理研究机构（ACRF）。NIAMS动物用户主要使用小鼠和大鼠。 NIAMS IRP动物项目监督了20项使用小鼠和大鼠模型的动物研究提案（ASP）。LACU协助研究人员准备ASP，并在NIAMS动物护理和使用委员会（ACUC）中有2名投票成员和1名候补成员。LACU为IRP维持寄养殖民地和哨兵计划。IRP项目领域包括关节炎、过敏性鼻炎和其他传递受体诱导的细胞反应、使用AID转基因小鼠的重组/染色质重塑、家族性地中海热（FMF）和TNF受体相关的周期性综合征。研究涉及肌肉和相关疾病（实验：N-RAP蛋白，糖原累积病II型，SIRT 1基因，肌原纤维组装和肌炎）和亚细胞器和骨骼肌中的细胞骨架，以及体外实验检查逆转录病毒转导，骨髓移植和使用胎儿和成人皮肤组织的表皮分化。该研究所的许多研究涉及细胞凋亡和自身免疫，以及软骨研究，重点是组织工程软骨，软骨形成，GDF-F，COMP和骨关节炎。 LACU在10月举行的2013年美国实验动物科学协会全国大会上展示了一张海报，概述了在两个月内成功转移70系转基因小鼠的情况。10/9 C127动物机构也在为2014年AAALAC研究中心访视做准备。

The LACU assists the NIAMS IRP investigators with their research involving animals. Such animals are housed in shared animal facilities on the NIH campus, including the NIAMS LACU located in Building 10 room 9/C127, the the Ambulatory Care Research Facility (ACRF) in Buildings 6B, 10A, 50 and Building 14. NIAMS animal users work primarily with mice and rats. The NIAMS IRP Animal Program has oversight for 20 Animal Study Proposals (ASPs) using mouse and rat models. The LACU assists researchers with preparing ASPs and has 2 voting members and 1 alternate on the NIAMS Animal Care and Use Committee (ACUC). The LACU maintains the foster colony and the sentinel program for the IRP. IRP project areas include arthritis, allergic rhinitis and other transmit receptor-induced cellular responses, recombination/chromatin remodeling using AID transgenic mice, familial Mediterranean fever (FMF), and TNF receptor associated periodic syndrome. Research involves muscle and related diseases (experimentation with: N-RAP protein, glycogen storage disease Type II, SIRT1 gene, myofibril assembly, and myositis) and sub-cellular organelles and cytoskeleton in skeletal muscle, as well as in vitro experiments examining retroviral transduction, bone marrow transplantation, and epidermal differentiation using fetal and adult skin tissues. Many of the institutes studies involve apoptosis and autoimmunity, as well as cartilage studies focused on tissue-engineered cartilage, chondrogenesis, GDF-F, COMP and osteoarthritis. The LACU is presenting a poster outlining the successful transfer of 70 lines of transgenic mice over a 2-month period at the 2013 American Association for Laboratory Animal Science National Convention in October. The 10/9C127 Animal Facility is also preparing for the 2014 AAALAC site visit.