Molecular Biology/Gene Expression Core (MBC)

核心C摘要 分子生物学/基因表达核心设施将提供关键服务，以支持科学 该计划项目的目标：1)分子试剂库-包含特定的质粒、表达结构、 报告构建、抗体、RNA样本和逆转录病毒/腺病毒/慢病毒载体 食道癌发生机制的研究。因此，这些试剂得到了组织和集中。在……里面 此外，我们将继续储存新的可用的试剂；2)图像分析和基因表达 定量-有多种仪器可用，例如(但不限于)磷光成像、凝胶和 Chemidoc、安捷伦生物分析仪和奥德赛红外蛋白质成像系统、实时定量聚合酶链式反应和 用于基因转录分析的发光仪、纳米液滴和流式细胞仪；3)基因组服务 (补贴)通过宾夕法尼亚微阵列设施使用基因微阵列(Affymetrix平台)，超高 RNA和CHIP-SEQ的DNA测序吞吐量和生物信息学支持；以及4)食道细胞系 银行(小鼠和人)具有原始的、永生的、转化的和新的基因工程 供项目使用的食道细胞系。分子生物学/基因表达核心提供高质量 保证、质量控制、成本效益、及时性和服务效率。这个核心是工作的 与所有其他核心，特别是分子病理学和成像核心(MPIC)密切合作，以避免 并为这些项目提供协调一致的办法。这些项目从中受益匪浅。 其核心在于提出相互关联的假设和具体目标。此外，此核心在中是动态的 响应不断发展的P01需求，预测未来P01的发展方向，并整合新兴技术。

CORE C ABSTRACT The Molecular Biology/Gene Expression Core Facility will provide critical services to support the scientific goals of this Program Project: 1) Molecular Reagent Bank- contains specific plasmids, expression constructs, reporter constructs, antibodies, RNA samples, and retroviral/adenoviral/lentiviral vectors of relevance to the study of esophageal carcinogenesis. As a result, these reagents have been organized and centralized. In addition, we will continue to store newly available reagents; 2) Image Analysis and Gene Expression Quantification-multiple instruments are available, such as (but not limited to) Phosphorimaging, Gel and Chemidoc, Agilent Bioanalyzer and Odyssey Infrared Protein Imaging System, real-time quantitative PCR, and Luminometers for gene transcription analysis, a nanodrop, and flow cytometer; 3) Genomic Services (subsidized) with gene microarrays (Affymetrix platform) through the Penn microarray facility, ultra-high throughput DNA sequencing for RNA- and CHIP-seq, and bioinformatics support; and 4) Esophageal Cell Line Bank (murine and human) with primary, immortalized, transformed and newly genetically engineered esophageal cell lines for use by the Projects. The Molecular Biology/Gene Expression Core provides quality assurance, quality control, cost-effectiveness, timeliness and efficiency in its services. This Core works closely with all the other Cores, especially the Molecular Pathology and Imaging Core (MPIC) to avoid duplication and furnish coordinated approaches for the Projects. The Projects benefit tremendously from this Core in advancing their interrelated hypotheses and Specific Aims. In addition, this Core is dynamic in responding to evolving P01 needs, anticipating future P01 directions, and integrating emerging technologies.