Facility Core: Small Molecule Mass Spectrometry Facility for Analytical & Prep.

设施核心摘要 小分子质谱学设施核心(SMMS)能够实现卤代有机化合物(HQCs)的高分辨率检测和分离，是该中心研究任务不可或缺的一部分。 该核心不复制斯克里普斯海洋学研究所(SIO)目前提供的任何分析服务或设施。SMMS设施核心将由P3领导人Aluwihare领导，设施的技术监督将由联席主任苏永轩博士提供。日常操作和例行维护将由Matthew Woolery先生负责。该设施将包括几个配置独特的气相色谱-质谱仪(GC-MS)，它们紧密集成在一起 该中心的研究使命是确定海洋环境中积累的一套HOCs，发现野生动物和人类中新的令人关注的HOCs，并评估HOCs的环境来源。PI的总体目标是确定与南加州海湾临时生产相关的生物体和栖息地。将通过核心提供能够筛选这些样品以进行相关HOC生产的GC电子捕获检测器(|JECD)/MS。项目2旨在确定和 描述海洋环境中多溴HOC生物合成途径的流行情况。因此，P2将依靠相同的仪器来筛选培养物，并确保被调查的生物产生感兴趣的化合物。P3的总体目标是确定可通过海鲜消费进入人类群体的小型、天然HOC套件。这项研究将得到整合到拟议设施中的两台GCxGC仪器的分析能力的支持。 如果没有全面的GCxGC分离，生物积累在顶端捕食者和人类体内的&gt；200 HOCs不能从共洗脱化合物中充分分离出来，以进行最终的鉴定和定量。为了确定所关注的HOCs的生物起源，P3还将对从顶端捕食者分离、纯化和浓缩以进行同位素测量的纯化合物进行稳定的同位素研究。设施中包括的独特配置的制备GCxGC仪器将支持这项研究。两台液质联用(LC-MS)仪器也将被安置在核心内，用于分析发酵提取物和合成有机制剂。内部咨询委员会将在每月会议期间讨论设施的业务，外部咨询委员会将每年评价设施核心的业绩。

Facility Core Summary The Facility Core for Small Molecule Mass Spectrometry (SMMS) is integral to the Center's research mission by enabling high-resolution detection and isolation of halogenated organic compounds (HQCs). This Core does not replicate any analytical services or facilities currently available at the Scripps Institution of Oceanography (SIO). The SMMS Facility Core will be directed by P3 Leader Aluwihare, and technical oversight for the facility will be provided Co-Director Dr. Yongxuan Su. Daily operation and routine maintenance will be the responsibility of Mr. Matthew Woolery. The facility will include several uniquely configured gas chromatography-mass spectrometry (GC-MS) instruments that are intimately integrated with the Center's research mission to identify the suite of HOCs accumulating in the marine environment, discover new HOCs of concern in wildlife and humans, and evaluate environmental sources of HOCs. The overall goal of PI is to identify organisms and habitats of interest with respect to HOC production in the Southern California Bight. A GC Electron Capture Detector (|JECD)/MS capable of screening these samples for relevant HOC production will be available through the Core. Project 2 aims to identify and characterize the prevalence of polybrominated HOC biosynthetic pathways in the marine environment. As such, P2 will rely on the same instrumentation to screen cultures and insure that organisms under investigation are producing compounds of interest. The overall goalof P3 is to identify the suite of small, natural HOCs available to enter human populations through seafood consumption. This research will be supported by the analytical capabilities of two GCXGC instruments integrated into the proposed Facility. The >200 HOCs that bioaccumulate in apex predators and humans cannot be adequately separated from co-eluting compounds for definitive identification and quantification without comprehensive GCXGC separation. To establish the biogenic origin of HOCs of interest, P3 will also conduct stable isotope studies on pure compounds that are isolated from apex predators, purified and concentrated for isotope measurement. A uniquely configured, preparative GCXGC instrument that is included in the Facility will support this research. Two liquid chromatography-mass spectrometry (LC-MS) instruments will also be housed in the Core to analyze fermentation extracts and synthetic organic preparations. The Internal Advisory Committee will discuss Facility business during monthly meetings, and the External Advisory Committee will evaluate the Facility Core for its performance annually.