Bioanalytical CoreBioanalytical Core

项目总结（见说明）：生物分析核心（核心C）服务于奥布莱恩中心社区，无论是在UAB和UCSD和扩展的研究基地，通过提供最先进的生物能量学，氧化应激分析和代谢物分析支持急性肾损伤（阿基）研究。核心C提供了一个全面的资源，包括优化的协议和技术，用于氧化应激和细胞生物能量学的生物分析，阿基的生物标志物，翻译后修饰和小分子生物化学的变化。这些服务包括咨询、实验设计培训、回收适合分析的样品和化验性能。提供的新服务包括确定线粒体生物能量学的独特技术，基于LC-质谱的肌酐，F2-异前列烷和柠檬酸循环中间体的测定。正在开发最先进的nanoLC-MS方法，以在临床样品和阿基动物模型中进行靶向和非靶向代谢组学和外泌体分析。核心C已经成功地支持肾脏研究界。自核心C启动以来，已为48名主要研究者进行了8,500多次检测，涉及56个项目。在48名调查员中，44名是非核心人员。每年使用核心C的研究者人数正在增加，年度出版率也在增加。核心C还支持了7个试点和可行性赠款获奖者的研究工作。这些共同努力目前已在23份同行审查的出版物中得到承认。核心将参与对调查人员的教育和培训，提供实践经验和科学交流。从2013年至2017年的一个经常性的特点将是一个为期4天的代谢组学培训研讨会（由NIGMS资助，作为NIH代谢组学共同基金计划的一部分）。最后，生物分析核心将与奥布莱恩中心领导层合作，确定研究者的新生物分析需求，并为中心实施这些需求。

PROJECT SUMMARY (See instructions): The Bioanalytical Core (Core C) serves the O'Brien Center community, both at UAB and UCSD and the extended research base by providing state-of-the-art bioenergetics, oxidative stress analysis and metabolite analysis support for acute kidney injury (AKI) research. Core C provides a comprehensive resource that includes optimized protocols and technology for bioanalytical analyses of oxidative stress and cellular bioenergetics, biomarkers of AKI, post-translational modifications, and changes in small molecule biochemistry. The services involve consultation, training in experimental design, recovery of samples suitable for analysis and assay performance. New services offered include unique techniques to determine mitochondrial bioenergetics, LC-mass spectrometry based assays for creatinine, F2-isoprostanes and citric acid cyle intermediates. State-of-the-art nanoLC-MS methods are being developed to perform both targeted and untargeted metabolomics and exosome analysis in clinical samples and from animal models of AKI. Core C has been successful in supporting the kidney research community. Since the inception of Core C, more than 8,500 assays have been performed for 48 principal investigators involving 56 projects. Of the 48 investigators, 44 were non-core personnel. The number of investigators using Core C each year is increasing, as is the annual publication rate. Core C has also supported the research efforts of 7 Pilot and Feasibility grant awardees. These combined efforts have been currently recognized in 23 peer-reviewed publications. The Core will participate in education and training of investigators providing hands-on experience and scientific interchange. A recurring feature from 2013-2017 will be an annual 4-day workshop for training in metabolomics (funded by NIGMS as part of the NIH Common Fund program in metabolomics). Finally, the Bioanalytical Core, in concert with the O'Brien Center leadership, will identify new bioanalytical needs of investigators and implement them for the Center.