Solution-Phase Biomolecular NMR at 24 T Fields Using New High Temperature Superco

描述(由申请人提供)：核磁共振是学术和工业生物医学界的核心。核磁共振的进一步进展受到了自20世纪70年代以来用于核磁共振/磁共振磁体的低温超导(LTS)导线的极限限制的挑战，这些限制阻止了它们以持续的非驱动模式在24T以上运行。这项提议建立在最近一项基于NHMFL的发展的基础上，该发展已经提供了第一个在e34T场以稳定超导状态工作的线圈，并建议利用这一突破来建立一个工作在1 GHz以上的完全超导溶液核磁共振系统。为了实现这一点，并证明利用这些想法将生物分子溶液态核磁共振携带到这个和更高(H30T)磁场中的可行性，我们的目标是：(I)开发一种高均匀度的圆形多丝Bi2212丝线圈，这是Larbalstier等人在2012年开发的一种新型高温超导体(HTS)，与以前提出的HTS不同，HTS可以携带超过30T的大电流密度；(Ii)在以持续模式运行的现有牛津仪器的输出磁铁中操作该线圈，以在具有H1-2ppm的均匀度和相关的场不稳定性的10 mm直接电压上提供超过23.5T的室温磁场最佳点；(Iii)利用NHMFL在过去十年中积累的在磁场稳定和探头构造方面积累的专业知识，以证明在室温下使用具有H30 ppb稳定性和均匀性的这种设置的可行性，超过150？L样品；(4)作为NHMFL设施作用的一部分，将该系统置于整个核磁共振社区的部署中，以便将其用于顺磁、膜-蛋白质、细胞和蛋白质生物核磁共振研究，并就进一步寻求这一突破的方法获得批判性反馈。

DESCRIPTION (provided by applicant): NMR is central to both the academic and industrial biomedical communities. Further progress in NMR is challenged by ultimate limitations in the low-temperature superconductor (LTS) wires used in NMR/MRI magnets since the 1970s, that prevent them from operating beyond 24T in a persistent, non-driven mode. This proposal builds on a recent NHMFL-based development that has delivered the first coil operating in a stable superconducting state at fields e34 T, and proposes to use this breakthrough for building a fully- superconducting solution NMR system operating above 1 GHz. To achieve this, and to demonstrate the feasibility of exploiting these ideas to carry biomolecular solution-state NMR to this and at higher (H30T) magnetic fields, we aim to: (i) develop a high homogeneity coil of round multifilamentary Bi-2212 wire, a new kind of high-temperature superconductor (HTS) developed by Larbalestier et al in 2012 and that unlike previously proposed HTSs can carry a high current density beyond 30 T; (ii) operate this coil within an existing Oxford Instrument LTS outsert magnet running in persistent mode, to deliver a room-temperature magnetic field sweet spot in excess of 23.5 T over a 10 mm DSV with homogeneities and relevant field instabilities of H1- 2 ppm; (iii) exploit the expertise that over the last decade NHMFL has amassed in field stabilization and probe construction, to demonstrate the feasibility of using such a setup at room temperature with H30 ppb stabilities and homogeneities over 150 ¿L samples; (iv) place this system to the disposition of the NMR community at large as part of NHMFL's facility role, in order to exploit it for paramagnetic, membrane-protein, in cell and protein bioNMR research, as well as to get critical feedback on ways to further pursue this breakthrough.