Core B: Gene Transfer Vector Core

项目摘要/摘要 爱荷华大学的载体核心被整合到基因治疗项目中，目的是研究 肺部疾病。载体核心员工是基因转移发展的积极参与者 囊性纤维化中心以及神经生物学、心血管、癌症和黄斑的技术 变性中心。与来自不同学科的研究人员的互动允许交叉受精 矢量设计中的想法、技术进步和创新。向量核心的总体目标是 在与CF相关的基因转移技术方面为研究人员提供支持。这包括咨询、开发 新型载体、功能和纯度载体的协作测试，以及常规载体准备。核心员工 调查人员在设计和开发的各个阶段都保持着密切的联系。核心将提供 纯化和浓缩的重组腺病毒、腺相关病毒(AAV)和 慢病毒。载体核心还将提供对标准细胞系、表达质粒和 记者病毒。因此，核心作为基因转移研究的研究和开发设施，以及 例行病媒准备的服务设施。向量核心也有一个异常强大的国家 通过将病毒载体分发给爱荷华州以外的许多机构和公司而存在。

PROJECT SUMMARY/ABSTRACT The Vector Core at the University of Iowa is integrated into gene therapy projects directed at the study of diseases of the lung. The Vector Core staff are active participants in the development of gene transfer technologies for the Cystic Fibrosis Center as well as Neurobiology, Cardiovascular, Cancer, and Macular Degeneration Centers. The interaction with investigators from various disciplines allows for cross-fertilization of ideas, technical advancements, and innovations in vector design. The overall objective of the Vector Core is to support investigators in gene transfer technologies relevant to CF. This includes consultation, development of novel vectors, collaborative testing of vectors for function and purity, and routine vector preparations. Core staff and investigators are in close contact through all phases of design and generation. The Core will provide purified and concentrated preparations of recombinant adenovirus, adeno-associated virus (AAV), and lentivirus. The Vector Core will also provide access to standard cell lines, expression plasmids, and stocks of reporter viruses. Thus, the Core serves as a research and development facility for gene transfer studies, and a service facility for routine vector preparations. The Vector Core also has an exceptionally strong national presence by distributing viral vectors to many institutions and companies outside of Iowa.