FACSAria Fusion Cell Sorter

 描述(由申请者提供)：我们申请资金购买一个高性能的细胞分选机和一个集成的生物安全柜(BSC)，以支持科罗拉多大学博尔德分校(UCB)的研究人员的创新研究。Becton，Dickinson and Company(BD)FACSAria Fusion是一款最先进的高性能细胞分选机，能够在由Baker公司设计的定制生物安全柜中实现多色分析器式灵敏度，以优化性能和安全性。拟议的仪器将取代核心设施中已连续运行16年的MoFlo Legacy分拣机。由于部件不可用和服务终止，该单元不能再 运行可靠，不久将需要退役。在过去的几年里，CU-Boulder在生物医学研究领域取得了显著的增长。在过去的十年中，荧光技术和细胞生物学的创新极大地增加了对细胞分选能力的需求。由于MoFlo Legacy分拣机的陈旧和严重的硬件限制，我们的核心设施在满足我们用户的需求方面已经远远落后。这些缺陷阻碍了核心设施的发展，以满足位于JSCBB和整个博尔德科学界的NIH资助的众多研究人员日益增长的需求。拟议的设备将使我们能够：1)继续为我们现有的核心用户群提供高质量的服务；2)满足尚未满足的对病原体和传染病样本进行分类和分析的研究需求。我们大楼和校园内的许多实验室都在进行这方面的研究，而在大博尔德地区没有细胞分选机能够或适当地在生物安全级别(BSL)2+或3进行分选。这一规定对于本提案中包含的研究目标的安全运行至关重要；3)扩展我们的能力，以服务于从事细菌和酵母工作的研究人员。目前我们是校园内唯一为这一群体用户服务的核心设施；4)提高我们的多色细胞分选和荧光模式(如FRET)的能力。我们的Legacy分选机只能在两个波长进行分选，校园内或附近没有具有445 nm和561 nm激光激发能力的细胞分选机，这是使用青色和/或红色荧光蛋白的构建的理想选择。我们迫切需要用BD FACSAria Fusion取代我们的现有技术，以继续支持CU-Boulder由NIH资助的广泛的尖端生物医学研究。有了新的仪器和能力，它将使用户能够在他们的研究中推动新的边界，并刺激创新。

 DESCRIPTION (provided by applicant): We request funds to purchase a high-performance cell sorter coupled with an integrated biological safety cabinet (BSC) to support innovative research by investigators at the University of Colorado-Boulder (UCB). The Becton, Dickinson and Company (BD) FACSAria Fusion is a state-of-the-art high-performance cell sorter capable of multicolor, analyzer-like sensitivity in a custom-fitted biological safety cabinet, designed by The Baker Company, for optimized performance and safety. The proposed instrument will replace a MoFlo Legacy sorter that has been in continuous operation for 16 years in the core facility. Due to the unavailability of parts, and termination of service, the unit can no longer be reliably operated and will soon need to be retired. The past several years CU-Boulder has seen significant growth in the field of biomedical research. Innovations in fluorescence technologies and cell biology during the past decade have dramatically increased the demand of cell sorting capabilities. Our core facility has fallen far behind in meeting the needs of our users due to the age and severe hardware limitations of the MoFlo Legacy sorter. These deficiencies hamper the growth of the core facility to serve the growing requirements of the numerous NIH funded researchers located within JSCBB and throughout the Boulder scientific community. The proposed equipment will allow us to: 1) continue to provide quality services to our existing core user base; 2) address unmet research need to sort and analyze pathogenic and infectious specimens. A number of laboratories in our building and on campus conduct research in this area and there is no cell sorter in the greater Boulder area that has the ability or proper enclosure to sort at biosafety level (BSL) 2+ or 3. This provision is critical for the safe operatin of research goals contained in this proposal; 3) expand our capabilities to serve investigators working on bacteria and yeast. Currently we are the only core facility on campus that serves this group of users; 4) increase our capabilities for multicolor cell sorting and fluorescence modalities such as FRET. Our Legacy sorter can only sort in two wavelengths and there is no cell sorter on campus or nearby with 445 nm and 561 nm laser excitation capabilities, which are ideal for constructs that utilize Cyan and/or Red fluorescent proteins. The replacement of our existing technology with the BD FACSAria Fusion is sorely needed to continue supporting the broad range of NIH funded cutting-edge biomedical research at CU- Boulder. With the new instrument and capabilities, it will empower the users to push new boundaries in their research and spur innovation.