OMICS CORE

高通量组学分析平台是分析复杂混合物的首选技术 来源于组织和生物基质，如肝脏、粪便、肠道、血液和尿液。为了最大限度地利用我们的 破译肝损伤的机制和治疗的能力、营养和肠道：肝的相互作用，以及 肝脏：环境/毒素/药物的相互作用，我们建议在肝脏生物学和毒理学中建立OMICS核心 科布雷提案。我们的OMICS核心由三个主要组成部分组成，包括代谢组学核心， 蛋白质组学核心和生物信息学。OMICS核心的总体目标是支持拟议的 科布雷研究公司。这一目标将通过应用最先进的科学和质量分离来实现 分析含有代谢物、蛋白质复杂混合物的研究样品的光谱分析技术 和蛋白质片段。这一目标是通过以下目标实现的： 具体目标1：液质联用(LC-MS)的应用，全面的两个 二维气相色谱飞行时间质谱仪和集成激光 消融电感耦合等离子体质谱(LA-ICPMS)平台定量分析 生物样品中的代谢物、金属离子和金属-蛋白质络合物。 具体目标2：高分辨率多维液相色谱质谱技术的应用 (MDLC-MS)和亲和富集法用于靶向蛋白质的定量。

High-throughput 'omics analysis platforms are the technologies of choice for analyzing complex mixtures derived from tissues and biological matrices such as liver, feces, intestine, blood and urine. To maximize our capability for deciphering the mechanism and therapy for liver injury, nutrition and gut:liver interactions, and liver:environment/toxin/drug interactions, we propose an OMICS Core in this Hepatobiology and Toxicology COBRE proposal. Our OMICS Core is composed of three major components including Metabolomics Core, Proteomics Core, and Bioinformatics. The overarching goal of the OMICS Core is to support the proposed COBRE research. This goal will be met through application of state of the art separations science and mass spectrometry techniques to analyze research samples containing complex mixtures of metabolites, proteins and protein fragments. This goal is achieved through the following aims: Specific Aim 1: Application of liquid chromatography mass spectrometry (LC-MS), comprehensive two- dimensional gas chromatography time-of-flight mass spectrometry (GCxGC/TOF-MS) and integrated laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) platforms for quantitative analysis of metabolites, metal ions, and metal-protein complex in biological samples. Specific Aim 2: Application of high-resolution multidimensional liquid chromatography mass spectrometry (MDLC-MS) and affinity enrichment methods for targeted protein quantification.