DNASU, the Plasmid Materials Repository, 2020 and Beyond

项目摘要 DNASU是一个非营利性的知识库，为学术界、政府和工业界提供基于DNA的 资源，其包括各种各样的质粒，包括专门的实验载体，调节载体， 基因序列和1,100多种生物的基因。储存在DNASU的质粒需要显著的 时间和成本的生产，和DNASU寻求避免重复的努力，并通过共享加速研究 他们公开。此外，DNASU安全地将质粒存档，并收集每个质粒的详细注释。 统一和易于访问的格式。用户通过我们的网站https：//dnasu.org/访问存储库， 在那里他们可以查看相关的克隆、基因和载体信息，以及与外部资源的链接 例如协议和出版物。DNASU通过我们自己的内部生产获得质粒 高通量克隆管道和通过沉积由外部研究小组，包括ORFeome 协作，这是一个多国合作，寻求建立一套完整的人类基因， 分享它们。此外，我们还与蛋白质结构倡议组织（PSI）合作，为他们进行了全面的研究。 在保藏之前对来自PSI中心的质粒进行长度测序和注释。所有质粒都是 存储在自动化存储检索系统（ASRS）中作为工作集合，并存储在单独的位置- 80 ° C冷冻箱用于存档储存。当用户访问DNASU查找并请求 通过互联网选择的结构。电子请求被传递到ASRS，ASRS检索克隆 对于传代培养和运输甘油储备液的技术人员，只需支付象征性的再收费。DNASU认为， 超过219，000个独特的质粒，并且还在不断增长。DNASU网站的平均点击率为9,000次， 每月收到155份订单，分发650多个质粒。我们已经运送了超过300，000个样品到 科学家分布在50个国家/地区，其中约80%在北美。 DNASU通过PSI倡议获得了NIGMS的资助，该倡议使其增加了> 92，000人 质粒到其收集和软件工具的开发定位和使用克隆，从而创建一个 全球独一无二的资源。在接下来的步骤中，我们将努力利用以下方面实现自我可持续性 解决DNASU操作和维护方面的四个具体目标：1）更新和现代化 DNASU软件符合当前规范; 2）完成人类ORFeome收集，以建立第一个 完善人类基因组的收集; 3）加强质粒的维护、存档和规范操作 确保最高质量控制的程序;以及4）建立营销计划，以维护社区 连接和提高DNASU作为科学家常规工具的意识。我们将结合使用 调整我们的再收费和增加分配，以满足我们的财务目标，并建立一个 咨询委员会，就我们的进展提供有用的反馈。我们从这个PAR寻求资金，以协助这一点， 计划建立自给自足，使DNASU可以继续作为一个资源，在未来许多年。

Project Summary DNASU is non-profit repository that provides academia, the government and industry with DNA-based resources, which comprise a wide variety of plasmids including specialized experimental vectors, regulatory genetic sequences, and genes from over 1,100 organisms. The plasmids stored at DNASU required significant time and cost to produce, and DNASU seeks to avoid duplicative effort and to accelerate research by sharing them openly. In addition, DNASU safely archives the plasmids and gathers detailed annotation for each in a uniform and easily accessible format. Users access the repository through our website, https://dnasu.org/, where they can view pertinent clone, gene and vector information, as well as link-outs to external resources such as protocols and publications. DNASU acquires plasmids both through in-house production by our own high throughput cloning pipeline and through deposition by outside research groups, including the ORFeome Collaboration, which is a multinational collaboration that seeks to build a complete set of human genes and share them. In addition, we partnered with the Protein Structure Initiative (PSI), for whom we performed full- length sequencing and annotation on plasmids from PSI centers before depositing them. All plasmids are stored in both an automated storage retrieval system (ASRS) as a working collection and in separately located - 80⁰ C freezers for archived storage. Distribution is initiated when a user visits DNASU to find and request selected construct(s) via the Internet. The electronic request is passed to the ASRS, which retrieves the clones for a technician who subcultures and ships the glycerol stocks for a nominal re-charge fee. DNASU maintains over 219,000 unique plasmids, and is constantly growing. The DNASU website sees an average of 9,000 hits, receives 155 orders and distributes over 650 plasmids per month. We have shipped >300,000 samples to scientists in 50 countries, with ~80% of these to North America. DNASU received funding from NIGMS through the PSI initiative that enabled the addition of >92,000 plasmids to its collection and the development of software tools for locating and using clones, thus creating a unique worldwide resource. For the next steps, we will strive to achieve self-sustainability utilizing the following four specific aims that address the operation and maintenance facets of DNASU: 1) Update and modernize DNASU software to current specifications; 2) Complete the Human ORFeome collection to establish the first complete human gene set collection; 3) Enhance our plasmid maintenance, archives and standard operating procedures to ensure the highest quality control; and 4) Establish a marketing program to maintain community connectivity and increase awareness of DNASU as a routine tool for scientists. We will use a combination of adjustments to our re-charge fees and increased distribution to meet our financial goals and establish an advisory board to provide useful feedback on our progress. We seek funding from this PAR to assist in this plan to establish self-sufficiency so that DNASU can continue as a resource for many years to come.