Neuroscience and Animal Models (NAM) Core

神经科学和动物模型核心（NAM）核心既是TMARC的资源，也是 将临床观察转化为适当的体外和体内实验的科学平台 研究与甲基苯丙胺（METH）/HIV诱导的脑相关疾病机制的模型 损伤 核心资源目标包括：1）提供转基因（gp 120和ITat）动物， 与HIV相关的神经系统（CNS）病理学; 2）为HIV的分子分析提供基础设施， 暴露于METH的HIV转基因动物中METH/HIV相关脑损伤的病理基质; 此外，通过与加州神经艾滋病组织网络（CNTN）的连接，NAM核心还将 访问库存的脑组织，以支持交叉验证动物和人类的翻译目标 最后，对于体外研究，我们将提供基于主要神经病理学的实验系统。 人神经胶质细胞培养物、神经元细胞系和脑血管平滑肌细胞; 3）Foster 翻译研究的想法，通过支持试点研究和培训领域的实验 神经病理学的年轻研究者。 核心科学目标包括：1）分析HIV中METH暴露引起的神经病理学 2）设计通过免疫组织化学方法储存的HIV大脑的神经病理学分析。 CNTN专注于与METH相关的衰老、血管和免疫介导的病理学; 3）通过 在试点研究的支持下，NAM核心将为未来的发展项目提供初步数据 在TMARC内，专注于慢性脑炎症、代谢和氧化应激、衰老等问题， METH对HIV转基因动物社会认知的影响， 神经保护干预。

The Neuroscience and Animal Models Core (NAM) Core serves both as a resource for TMARC and as a scientific platform for translating clinical observations into appropriate in vitro and in vivo experimental models to study mechanisms of disease associated with methamphetamine (METH)/HIV-induced brain injury. The Core Resource Objectives include: 1) Provide transgenic (gp120 and ITat) animals that exhibit central nervous system (CNS) pathology associated with HIV; 2) Provide the infrastructure for molecular analysis of the pathologic substrates in METH/HIV associated brain injury in HIV transgenic animals exposed to METH; furthermore, through linkage to the California NeuroAIDS Tissue Network (CNTN), the NAM Core will also access banked brain tissues that will support the translational aim of cross validating animal and human neuropathology, and finally, for in vitro studies we will provide experimental systems based on primary human neuroglial cultures, neuronal cell lines, and brain vascular smooth muscle cells; 3) Foster translational research ideas by supporting Pilot studies and training in the field of experimental neuropathology for young investigators. The Core Scientific Objectives include: 1) Analyze the neuropathology induced by METH exposure in HIV transgenic animals, young and aged; 2) Design neuropathologic analyses of HIV brains banked through the CNTN focused on aging, vascular and immune-mediated pathology associated with METH; 3) Through support of Pilot studies, the NAM Core will generate preliminary data for future developmental projects within TMARC focused on issues such as chronic brain inflammation, metabolic and oxidative stress, aging and brain vascular pathology, METH effects on social cognition in HIV transgenic animals, and neuroprotective interventions.