High resolution Thermo Scientific Q-Exactive Orbitrap mass spectrometer for metabolomics

项目摘要 这里建议购买热科学混合四极轨道Q Exactive PLUS质谱仪与Thermo Science Vanquish UHPLC系统联用 发现和靶向代谢组学分析。所请求的仪器将支持大型 NIH资助的调查人员数量，包括六个主要用户和六个次要用户，正在进行 代谢组学项目。这些项目的重点不同，从癌症、糖尿病、肥胖到年龄- 相关的眼部疾病。此外，糖尿病培训和研究中心的调查人员 中心、消化疾病中心、范德比尔特·英格拉姆癌症中心和范德比尔特 视觉研究中心将从新功能中受益。Q Exactive Plus系统将 放在范德比尔特质谱学的小分子质谱学核心中 研究中心。该仪器将由核心的四名专业人员组成的团队管理 包括负责培训用户的副主任、负责 仪器QA/QC和维护，负责仪器维修的工程师和PI 负责仪器设备的监督。业务资金将来自核心的 范德比尔特研究办公室制定并经批准的标准收费表 核心的监督委员会。此外，还将设立一个文书咨询小组 并由主要用户、机构核心管理的成员和 代谢组学专家。该仪器的主要目的将是取代8-10岁的孩子 并提供最先进的灵敏度和整体性能，以增强 12名研究人员和三个主要中心的研究计划。

Project Summary Proposed here is the purchase of a Thermo Scientific hybrid quadrupole-orbitrap Q Exactive Plus mass spectrometer coupled with a Thermo Scientific Vanquish UHPLC system for discovery and targeted metabolomics analyses. The requested instrument will support a large number of NIH funded investigators including six major users and six minor users with ongoing metabolomics projects. These projects vary in focus from cancer, diabetes, obesity, to age- related eye diseases. Furthermore, investigators from the Diabetes Training and Research Center, the Digestive Disease Center, the Vanderbilt Ingram Cancer Center, and the Vanderbilt Vision Research Center will benefit from the new capabilities. The Q Exactive Plus system will be placed in the small molecule Mass Spectrometry Core of the Vanderbilt Mass Spectrometry Research Center. The instrument will be managed by a team of four professionals in the Core including the Associate Director responsible for training users, a technician responsible for instrument QA/QC and maintenance, an engineer responsible for instrument repair, and the PI responsible for instrument oversight. Operational funds will be generated from the Core's standard fee schedule as established by the Vanderbilt Office of Research and as approved by the Core's oversight committee. In addition, an instrument advisory panels will be established and consist of major users, a member of the institution's Core administration, and a metabolomics expert. The primary purpose of the instrument will be to replace 8-10 year old instruments and to provide state-of-the-art sensitivity and overall performance to enhance the research programs of twelve investigators and three major centers.