Microscopy and Imaging Center

摘要 成像技术是发挥创造力和力量的关键工具 在现代肾脏研究的许多方面。参与这项研究的肾脏研究人员 计划项目赠款要求随时获得高质量的成像设备和服务。 现有的显微镜和成像中心核心设施就是为满足这一需求而设计的。 核心的结构是将来自几个国家的资源和专业知识结合起来 将大学内的不同地点合并为一个统一的实体，作为一个整体 设计和执行成像研究的合作伙伴。该设施包括全系列的高 优质成像设备，包括：荧光显微镜、荧光成像 系统，新的激光扫描共聚焦显微镜，旋转圆盘共聚焦显微镜，和 电子显微镜和许多用于组织切片和样品制备的设备。这个 CORE的技术人员展示了数十年在成像方法方面的综合经验 和技术发展。监督人员包括两名资深科学家，他们都是 在肾脏和肾脏的各种成像方式的应用方面有丰富的专业知识 生理学研究。该团队将共同努力，以实现以下目标：1) 为PPG小组研究人员提供高质量的成像设备，包括 荧光显微镜、激光扫描共聚焦显微镜、实时成像 细胞和小管的生理参数及电子显微镜；2)提供PPG组 具有光学和电子显微镜样品制备和处理专家的研究人员；3) 为PPG小组研究人员提供专家协助，帮助他们生成、解释和 静态图像的量化，包括记录器官结构、肾组织学和 肾脏蛋白质的亚细胞定位；以及4)为PPG小组研究人员提供 获得所需的设备和专业知识，以制作活细胞和 组织，包括器官结构的活体评估，细胞内离子的测量 浓度，以及蛋白质运输的实时观察。

Summary Imaging techniques constitute critical tools that are brought to bear with creativity and power in many aspects of modern renal research. The renal researchers who participate in this Program Project Grant require ready access to high quality imaging equipment and services. The existing Microscopy and Imaging Center core facility has been designed to meet this need. The core has been structured in a manner that unites resources and expertise from several different locations within the University into a single unified entity that can serve as an integral partner in the design and execution of imaging studies. The facility includes a full range of high quality imaging equipment, including: fluorescence photomicroscopes, fluorescence imaging systems, a new laser scanning confocal microscope, a spinning disk confocal microscope, an electron microscope and numerous devices for tissue sectioning and sample preparation. The technical staff of the core manifests decades of combined experience in imaging methodologies and technique development. The supervisory staff includes two senior scientists, both of whom have extensive expertise in the application of the various modalities of imaging to renal and physiological research. This team will work together to meet the following objectives: 1) to provide PPG group researchers with ready access to high quality imaging modalities, including fluorescence microscopy, laser scanning confocal microscopy, real time imagining of physiological parameters in cells and tubules and electron microscopy; 2) to provide PPG group researchers with expert sample preparation and processing for light and electron microscopy; 3) to provide PPG group researchers with expert assistance in the generation, interpretation and quantitation of static images, including those documenting organ structure, renal histology, and the subcellular localizations of renal proteins; and 4) to provide PPG group researchers with access to the equipment and expertise required to produce dynamic images of living cells and tissues, including in vivo assessment of organ structure, measurements of intracellular ion concentrations, and real time observations of protein trafficking.