Impact of prenatal insecticide exposure on neurodevelopmental trajectories in a Thai birth cohort: building exposure science and neurodevelopmental research capacity in Thailand

项目摘要 在发展中国家，接触杀虫剂是无处不在的，在那里从事务农活动的妇女 在整个怀孕期间经常暴露。尽管美国的几项出生队列研究表明 产前接触有机磷杀虫剂对神经发育的持续影响 暴露导致不良后果的神经发育途径和时间尚不清楚。因此， 监管机构一直不愿改变风险评估标准或提供具体的 关于孕妇接触的建议。建议的纵向出生队列研究 泰国将：1)建设泰国研究人员的能力，以开发创新和具有竞争力的农药研究 相关神经毒性2)测量OP和拟除虫菊酯(PYR)杀虫剂在每三个月期间的代谢物 怀孕，以及3)评估产前OP(和PYR作为探索性分析)杀虫剂暴露的影响 关于从出生到3岁的神经发育轨迹。与朱拉隆功大学合作 泰国中部和泰国北部的清迈大学(CMU)将招募300名孕妇。 在怀孕期间将采集8个连续尿样，4个连续血液样本和脐带血 记录每三个月期间临时解决的杀虫剂暴露情况。布拉斯尔顿神经行为 出生时将对后代进行评估量表，然后进行视觉注意力测试、调节 情绪、记忆和抑制控制，以确定神经发育的最终“级联” 影响整体认知功能(例如，贝利-III)。能力建设活动包括发展核心 CMU的分析实验室设施和CU的神经发育设施以及促进 资助写作和国际科学出版物。

Project Summary Exposure to insecticides is ubiquitous in the developing world, where women who perform farming activities throughout pregnancy are routinely exposed. Although several birth cohort studies in the U.S. have shown persistent neurodevelopmental effects of prenatal organophosphate (OP) insecticide exposure, the neurodevelopmental pathways and timing of exposure leading to adverse outcomes is not clear. Thus, regulatory agencies have been reluctant to change risk assessment standards or provide specific recommendations about exposures to pregnant women. The proposed longitudinal birth cohort study in Thailand will: 1) build capacity of Thai researchers to develop innovative and competitive studies of pesticide related neurotoxicity 2) measure metabolites of OP and pyrethroid (PYR) insecticides during each trimester of pregnancy, and 3) evaluate the impact of prenatal OP (and PYR as exploratory analysis) insecticide exposure on neurodevelopmental trajectories from birth to age 3. In collaboration with Chulalongkorn University (CU) in central Thailand and Chiang Mai University (CMU) in northern Thailand, 300 pregnant women will be recruited. Eight serial urine samples, 4 serial blood samples, and umbilical cord blood will be collected during pregnancy to document temporally-resolved insecticide exposure during each trimester. The Brazelton Neurobehavioral Assessment Scale will be administered to offspring at birth followed by tests of visual attention, regulation of emotion, memory and inhibitory control to determine how the “cascade” of neurologic development ultimately affects overall cognitive function (e.g., Bayley-III). Capacity building activities include development of core analytic laboratory facilities at CMU and neurodevelopmental facilities at CU along with courses to promote grant writing and international scientific publications.