Cellular Imaging Core

项目摘要：细胞成像核心 先进的细胞显微镜是生物学研究的有力工具，在 对疾病发病机制的研究，最终可能转化为新的治疗方法 风湿病。成像技术在过去的十年中迅速发展，带来了改进 在分辨率、灵敏度和速度方面，这为研究创造了根本的新机会 在活细胞和动物中，跨越许多数量级的实时过程。同时， 越来越复杂的设备的成本是巨大的，高效使用的专业知识， 维护和开发这种设备在大多数实验室中并不常见。因此，存在着显著的差距。 在这些强大工具的可用性和个人调查人员访问和使用的能力之间 他们效率很高。细胞成像核心是为了响应风湿性疾病的反馈而创建的 以资源为基础的研究中心成员利用在新的 创建了华盛顿大学细胞成像中心(WUCCI)，并提供了一种集成方法来 风湿病相关模型细胞和组织结构及动态行为的研究 系统。核心的总体目标是提供访问和技术支持，使用高级 细胞显微镜工具加快了风湿病治疗的步伐，扩大了范围，提高了效率 疾病研究。核心服务可满足众多调查人员的独特需求 基础研究和翻译研究的范围，吸引新的研究人员进入风湿病研究 区域。重要的是，用户受益于核心总监和技术人员的深入技术专长 工作人员在咨询中花费时间进行实验设计和数据解释。在过去的几年里 在WUCCI业务的财政年度(2016年7月1日至2017年6月30日)，核心为184个研究实验室提供服务， 其中11个是RDRRC成员，其使用量约占核心消费总量的10%。它是 预计蜂窝成像核心服务需求将继续增长 在风湿性疾病研究中需要先进的显微成像和图像分析方法。 细胞成像核心将提供基本服务，使之能够并加速研究 RDRRC的调查人员将在下一个资金周期内开展这项工作。

PROJECT SUMMARY: Cellular Imaging Core Advanced cellular microscopy is a powerful tool for biological research and has an important role to play in the study of disease pathogenesis, which may translate eventually to novel treatment approaches for rheumatic diseases. Imaging technology has evolved rapidly over the last decade leading to improvements in resolution, sensitivity and speed, which have created fundamentally new opportunities for studying processes across many orders of magnitude and in real-time in living cells and animals. At the same time, the costs of increasingly sophisticated equipment are substantial and the expertise to efficiently use, maintain, and develop this equipment is not common in most labs. There is, therefore, a significant gap between the availability of these powerful tools and the ability of individual investigators to access and use them efficiently. The Cellular Imaging Core was created in response to feedback from Rheumatic Diseases Research Resource-based Center members to leverage the significant institutional investment in the newly created Washington University Center for Cellular Imaging (WUCCI) and provide an integrated approach to investigate the structure and dynamic behavior of cells and tissues rheumatic disease-related model systems. The overall objective of the Core is to provide access to and technical support in using advanced cellular microscopy tools to accelerate the pace, expand the scope, and improve efficiency of rheumatic diseases research. The Core services meet the unique requirements of numerous investigators over a wide range of basic and translational research, attracting new investigators into rheumatic diseases research areas. Importantly the users benefit from the in-depth technical expertise of the Core Director and technical staff in the time spent in consultation for experimental design and interpretation of data. During the last fiscal year (July 1 2016 – June 30 2017) of WUCCI operations, the Core served 184 research laboratories, 11 of these were RDRRC members whose usage represented ~10% of the overall core consumption. It is anticipated that requests for Cellular Imaging Core services will continue to grow given the overwhelming need for advanced microscopic imaging and image analysis approaches in the study of rheumatic diseases. The Cellular Imaging Core will provide essential services that will enable and accelerate the research of RDRRC investigators throughout the next funding cycle.