Comprehensive Biology: Exploiting the Yeast Genome

项目摘要 自成立以来，酵母资源中心一直致力于了解基因组序列是如何关联的 对蛋白质的作用。该中心成立时，萌芽酵母成为第一个拥有其基因组的真核生物 序列完成，我们建议使用一系列技术来解释这个序列。在这 应用，我们承担了新的挑战，集中在了解蛋白质的变异如何影响它们的 层次、修饰、功能和结构。我们将在三个方面开发新技术：1)扰动和 感知复杂通路的变化；2)蛋白质的质谱学检测和定量；以及3) 高阶蛋白质结构。这些技术将由十项紧密整合的驱动生物医学技术推动 项目。酵母仍然是开发、测试和改进这些技术的无与伦比的实验系统。 随着技术的成熟，我们将通过内部项目和 外部合作。此外，我们通过以下方式使我们的技术在变得强大时可用 广泛的合作和良好的传播和培训记录。

Project Summary Since its inception, the Yeast Resource Center has focused on understanding how genome sequence relates to protein function. The Center began just as budding yeast became the first eukaryote to have its genome sequence completed, and we proposed to use an array of technologies to interpret this sequence. In this application, we undertake novel challenges, centered on understanding how variation in proteins affects their levels, modification, function and structure. We will develop new technologies in three areas: 1) Perturbing and sensing changes to complex pathways; 2) Protein detection and quantitation by mass spectrometry; and 3) Higher order protein structure. These technologies will be driven by ten closely integrated Driving Biomedical Projects. Yeast remains an unparalleled experimental system to develop, test and refine these technologies. As the technologies mature, we will extend their application to higher eukaryotes through internal projects and external collaborations. Furthermore, we make our technologies available as they become robust, through broad collaboration and a well-established track record of dissemination and training.