ShEEP Request for Keyence BZ-X800E All-in-One Fluorescence Microscope

项目摘要 Keyence BZ-X800E多功能一体荧光显微镜是一种独特的仪器， 提供多色荧光成像、3D图像渲染和测量定位、活细胞 和延时视频生成、自动多图像采集以及实时图像拼接和 图像分析。因此，Keyence显微镜将成为VAWNYHS的重要仪器 这些独特的功能将对以下退伍军人管理局资助的项目产生立竿见影的效果。 特罗恩博士的工作调查了老年人的肌肉骨骼老化、虚弱和功能能力 退伍军人。特罗恩博士正在进行人体和动物研究，以探索高血糖的益处。 大强度间歇训练和营养补充对骨骼肌和骨形态的影响 并在衰老过程中发挥作用。Keyence将允许更精确地评估肌肉纤维 是当前方法的时间的一小部分。Keyence还将能够产生延时 实时细胞成像视频观察骨吸收破骨细胞的形成和活性，捕捉 试剂干预的影响，并促进分析实验动物的骨骼。 Fliesler博士的项目研究进行性视网膜变性的潜在机制 和与冲击波超压引起的多发伤相关的视觉功能障碍，以及 作为治疗剂的新型抗氧化剂，可预防、减少或减缓糖尿病的进展 病理过程。Keyence显微镜将提供组织学的定量评估 氧化应激、细胞死亡和胶质细胞增多症的损伤和生物标志物。 沙利文博士的研究调查了转录后基因沉默试剂的发展 例如核酶(催化RNA)作为孤儿视网膜变性的候选治疗药物(例如： 视网膜色素变性)和常见的老年性黄斑变性(AMD)。基恩斯将 提供引人注目的新方法来观察细胞RNA运输并研究其动力学 以及催化RNA(核酶)疗法的稳定结果。 坎蒂博士的研究调查了心脏骤停和心脏骤停时的动态重构 干细胞介导的修复。Keyence将有助于表征特定的造血细胞 亚型和巨噬细胞的极化，使茎在培养中的特性更好 了解成体干细胞如何刺激内源性心肌细胞增殖。 冯博士的研究试图了解parkin基因突变是如何导致选择性退行性变的 人类黑质多巴胺能神经元和随之而来的帕金森氏病。Keyence将允许 监测IPSC衍生的中脑DA的形态变化和成像的能力 神经元，以确定帕金森突变作为特发性帕金森病模型的影响。 朗博士的研究试图确定外切体在干细胞介导的功能中的作用 心脏修复。Keyence显微镜将通过促进极高的 小鼠心肌梗死后心肌组织分辨免疫组织学分析 评估基于外切体的治疗改善受损组织的潜力。 Keyence BZ-X800E显微镜提供的应用范围将极大地促进 我们对导致生理学的潜在的急性和慢性过程的理解 功能障碍、疾病和健康下降。此外，它将是VAWNYHS的重要工具 研究人员开发有效的干预措施，以最大限度地减少严重程度，推迟发病，或阻止 这可能会导致退伍军人的身体虚弱，并最终提高他们的生活质量。

Project Abstract The Keyence BZ-X800E All-in-One fluorescent microscope is a unique instrument that provides multi-color fluorescent imaging, 3D image rendering and measurement localization, live cell and time-lapse video generation, automated multi-image collection, and real-time image stitching and image analysis. Thus, the Keyence microscope will be a crucial instrument for VAWNYHS investigators, and the unique features will have immediate benefits for VA-funded projects below. Dr. Troen’s work investigates musculoskeletal aging, frailty, and functional capacity in older veterans. Dr. Troen is conducting both human and animal studies to explore the benefits of high intensity interval training (HIIT) and nutritional supplements on skeletal muscle and bone morphology and function during aging. The Keyence will allow more precise assessments of muscle fibers in a fraction of the time of the current methodology. The Keyence will also be able to generate time-lapse live cell imaging video to observe the formation and activity of bone resorbing osteoclasts, capture the impacts of reagent interventions, and facilitate analysis of bones from experimental animals. Dr. Fliesler’s projects investigate the underlying mechanism of progressive retinal degeneration and visual dysfunction associated with blast overpressure-induced polytrauma, and the impact of novel antioxidants as therapeutic agents to prevent, minimize, or slow the progression of the pathological processes. The Keyence microscope will afford quantitative assessment of histological damage and biomarkers of oxidative stress, cell death, and gliosis. Dr. Sullivan’s study investigates the development of post-transcriptional gene silencing agents such as ribozymes (catalytic RNAs) as candidate therapeutics for orphan retinal degenerations (e.g. retinitis pigmentosa) and common age-related macular degeneration (AMD). The Keyence will provide dramatic new methodologies to observe cellular RNA trafficking and investigate the kinetics and steady-state outcomes of catalytic RNA (ribozyme) therapeutics. Dr. Canty’s study investigates dynamic remodeling during sudden cardiac arrest and cardiac stem cell mediated repair. The Keyence will facilitate characterization of specific hematopoietic subtypes and macrophage polarization and enable characterization of stems in culture to better understand how adult stem cells stimulate endogenous myocyte proliferation. Dr. Feng’s study seeks to understand how mutations of parkin cause the selective degeneration of human nigral dopaminergic neurons and the ensuing Parkinson’s disease. The Keyence will allow the ability to monitor morphological changes and enable imaging of iPSC-derived midbrain DA neurons, to identify impacts of parkin mutations as a model of idiopathic Parkinson’s disease. Dr. Lang’s research seeks to determine the functional role of exosomes in stem cell-mediated cardiac repair. The Keyence microscope will enhance this work by facilitating extremely high resolution immunohistological analysis of murine cardiac tissue following myocardial infarction and evaluating the potential of exosome-based therapy to ameliorate damaged tissues. The range of applications provided by the Keyence BZ-X800E microscope will greatly facilitate our understanding of the underlying acute and chronic processes that result in physiological dysfunction, disease, and health decline. Further, it will be an essential tool for the VAWNYHS researchers to develop effective interventions to minimize the severity, delay the onset, or arrest the progression of frailty and ultimately improve the quality of life in the veteran population.