Organoid and Minibioreactor Array Cultivation Core

项目总结 U19传染病基因组中心(GCID)应用程序的目标之一是使用最新的 ART平台，解决围绕多种NIAID A-C类细菌、病毒、真菌、 以及引起胃肠道、肠外和呼吸道疾病的寄生粘膜病原体。至 为了实现这一目标，有机和微型生物反应器阵列培养(OMAC)核心的建立将使 提供关键的生物学意义上相关的人体器官类型和细菌培养，这些培养对 项目的成功。核心将围绕三个目标进行组织：1)生成、维护和提供 实验准备好的肠道和呼吸道有机培养物和专门的生长试剂可供使用，(2) 从肠道和肺部培养各种类型的微生物群落；(3)建立和监督 利用厌氧平台进行实验，以促进微生物和有机物的共培养。这些目标将 提供通过体外验证获得的关键结果来转换基因组分析的能力 测序研究。核心将减少劳动力、供应和设备成本，并减少完成所需的时间 通过集中隔离、生长和维护类有机物和复合微生物的项目 社区。培养有机物和细菌所需材料的批量获取标准化 含有生长因子和生物反应器的条件培养基及集中维护 管线和生物反应器为各个项目调查员节省了大量的时间和成本 确保标准化和重现性。此外，OMAC核心将增强通信和 项目之间取得的成果的可比性。OMAC核心将是 这个应用程序中每个项目的成功以及将它们连接在一起的统一要素。

PROJECT SUMMARY One of the goals of this U19 Genomic Center for Infectious Disease (GCID) application is to use state-of-the- art platforms to address gaps in knowledge surrounding multiple NIAID Category A-C bacterial, viral, fungal, and parasitic mucosal pathogens that cause gastrointestinal, extraintestinal, and respiratory tract diseases. To meet this goal, the establishment of the Organoid and Minibioreactor Array Cultivation (OMAC) core will allow the provision of critical biologically relevant human organotypic and bacterial cultures that are essential for the success of the project. The core will be organized around three goals: 1) generate, maintain, and provide experimentally ready intestinal and airway organoid cultures and specialized growth reagents for use, (2) cultivate various types of microbial communities from the intestine and lung, and (3) establish and oversee experiments with an anaerobic platform to facilitate co-culture of microbes and organoids. These goals will offer the ability to translate genomic analysis through in vitro validation of key outcomes obtained from sequencing studies. The core will reduce labor, supply and equipment costs, and time required to complete projects by centralizing the isolation, growth, and maintenance of the organoid lines and complex microbial communities. Bulk acquisition of materials required to culture organoids and bacteria, standardization of conditioned media containing growth factors and bioreactor media, and centralization of the maintenance of the lines and bioreactors results in significant time and cost savings to the individual Project investigators and ensures standardization and reproducibility. Additionally, the OMAC core will enhance communication and comparability of results obtained between projects. The OMAC core will be an essential component of the success of each of the projects in this application as well as a unifying element in connecting them together.