TRD1

建议继续支持荧光动力学实验室扩大 技术生物医学研究的新领域的发现和应用，并加强 开展合作、服务、培训和传播活动， 研究社区。荧光成像和荧光方法学已经达到了 在生物学，生物物理学和临床研究中发挥着重要作用。LFD是一种生物技术 拥有成功开发新荧光技术的历史， 生物学和医学，并坚定地致力于服务，培训和传播 旨在将荧光方法引入用户设施， 生物医学研究社区。 对于这一建议，我们已经构思了一个强大的技术开发计划，以扩大目前的 成像荧光仪器的局限性，特别是对动态细胞的研究 流程.项目包括：开发用于深层成像的新型显微镜平台 组织，仍然保持衍射极限分辨率;利用光片显微镜， 波动分析，并有可能开发全新的方法来衡量 纳米级的细胞和组织中的分子流;使用新的电子和光学3D 细胞动力学成像;将相量方法扩展到荧光寿命成像 对光谱和扩散分析进行分析，以实现动态过程的快速、无拟合可视化 并扩展我们的软件，使新的数据采集和分析算法， 显微镜. LFD的组织结构允许用户和合作者访问创新的 技术只有在这个BTRR资源，这为他们提供了一个独特的机会， 迅速推进自己的研究计划。一个丰富的推动生物问题的计划 利用和推动生物过程、大分子 组装，组织组织和动力学，膜形态/功能关系，以及 用于检测血液中细菌的创新生物医学仪器， 在临床领域的影响。针对此应用程序提出的教育计划包括动手实践 培训学生、博士后研究员和访问科学家，举办讲习班和专门 学校在创新的荧光方法。我们改进后的网站将继续成为 关于荧光动态技术的信息的参考源，以及一个大型存储库 出版物、讲座和辅导。

Continuing support is proposed for the Laboratory for Fluorescence Dynamics to expand technological biomedical research to new fields of discovery and applications and to strengthen collaborative, service, training and dissemination activities for the benefit of the biomedical research community. Fluorescence imaging and fluorescence methodologies have reached a prominent role in Biology, Biophysics and in Clinical Research. The LFD is a biotechnological resource center with a history of successes in developing new fluorescence technologies for biology and medicine and with a strong commitment to service, training, and dissemination components aimed at bringing fluorescence methods in a user facility available to the biomedical research community. For this proposal we have conceived a strong technical development program to expand current limits of imaging fluorescence instrumentation, particularly for the study of dynamical cellular processes. Projects include: developments of new microscope platforms for imaging in deep tissues, still maintaining diffraction limited resolution; exploiting the light sheet microscope for fluctuation analysis with the potential for developing radical new approaches to measure molecular flow in cells and tissue at the nanoscale; using new electronics and optics for 3D imaging of cellular dynamics; expanding the phasor approach to fluorescence lifetime imaging analysis to spectral and diffusion analysis for fast, fit-free visualization of dynamical processes and expanding our software to allow new algorithms for data acquisition and analysis in our new microscopes. The organization structure of the LFD allows users and collaborators to access innovative technologies only available in this BTRR resource, which affords them a unique opportunity to rapidly advance their own research programs. A rich program of driving biological problems exploit and push technological advances and research in biological processes, macromolecular assembly, tissue organization and dynamics, membrane morphology/function relationships, and innovative biomedical instrumentation for detection of bacteria in blood which could have an impact in the clinical area. Educational programs proposed for this application include hands-on training of students, postdoctoral fellows and visiting scientists, workshops and specialized schools in innovative fluorescence methodologies. Our improved web site will continue to be a reference source for information about fluorescence dynamic techniques, and a large repository of publications, lectures and tutorials.