Enteroid Core - Pathogenesis of E. Coli and Shigella Infections in Human Enteroid Models

项目摘要 肠样核将从我们建立的JHU生物库向研究人员提供肠样/结肠样物质 P01作为冻干，说明如何培养和繁殖肠样，如何使用条件化 以及如何制作肠样/结肠样单层。将准备好有条件的介质并提供给 调查人员每周一次。与免疫学核心合作，肠样核心将准备静态免疫 细胞/肠样共培养模型表征接触细菌后的天然免疫反应 病原体(即EHEC、EAEC、ETEC和志贺氏菌)。共同文化模式将被开发为具有不同的 免疫细胞的类型包括巨噬细胞、粒细胞和树突状细胞。

Project Summary The Enteroid Core will provide enteroids/colonoids from our established JHU biobank to investigators of this P01 as frozen stocks, instruction given on how to culture and propagate the enteroids, how to use conditioned media and how to make enteroid/colonoid monolayers. Conditioned media will be prepared and provided to the investigators weekly. In collaboration with the Immunology Core, the Enteroid Core will prepare static immune cell/enteroid co-culture models to characterize the innate immune response after exposure to bacterial pathogens (i.e. EHEC, EAEC, ETEC and Shigella). The co-culture model will be developed to feature various types of immune cells including macrophages, granulocytes, and dendritic cells.