Core Facility for Aged Rodents

摘要 老年啮齿动物核心设施（CFAR）的主要目标是开发新的小鼠模型， 用于评估控制衰老速度和将衰老与晚年联系起来的生理和细胞因素 疾病新的小鼠模型经常由其他科学家，在UM或其他机构提出，这些 被认为是潜在的合作。CFAR资源通常用于初始表征， 包括对寿命、对年龄敏感的健康指数和病理学的研究， 足够的数据，使新的小鼠模型用于竞争性应用的校外支持。在 与UM OAIC的总体主题保持一致，许多开发的鼠标系统依赖于改变 代谢和/或炎症途径，无论是通过遗传变化，饮食干预，或更多 最近，毒品。每年选择的特定突变体、饮食或药物取决于科学发现， 每一年的互动。具体目标：目标1将支持六个外部资助项目， 第一年，包括对5-羟色胺受体突变体的研究，药物对基因表达模式的影响，抗 针对癌细胞的抗衰老药物，特定mRNA的非帽依赖性翻译工作，脂肪组织研究 炎症，并分析保护线粒体免受氧化损伤的酶的过度表达 损害目的2利用CFAR资源维持对小鼠衰老特别感兴趣的繁殖群体 研究，包括生长激素受体组织特异性改变的突变体，IGF 1结合 蛋白质蛋白酶PAPPA、线粒体氧化还原保护酶硫氧还蛋白还原酶2和两种蛋白质 其促进mRNA亚群的帽非依赖性翻译。目标3侧重于培训、咨询和 专业教育，使CFAR可以提供先进的观点，小鼠衰老模型，以PES 和REC应用程序，并UM学生，研究员和教师，并有助于国家的发展 啮齿类动物最佳使用标准。目标4涉及选择一个或两个新的创新 小鼠模型的发展，在每一年的奖项。CFAR自1989年成立以来， 地方和国家领导开发新的小鼠模型，用于控制 老龄化及其与老年疾病的联系，并希望在下一个奖项期间保持这一作用。

Abstract The principal goal of the Core Facility for Aged Rodents (CFAR) is to develop new mouse models that can be used to evaluate the physiological and cellular factors that control the rate of aging and tie aging to late-life diseases. New mouse models are often suggested by other scientists, at UM or at other institutions, and these are evaluated as potential collaborations. CFAR resources are used for initial characterizations, often including studies of lifespan, age-sensitive indices of health, and pathology, with a view towards producing sufficient data to allow the new mouse models to be used in competitive applications for extramural support. In keeping with the overall theme of the UM OAIC, many of the mouse systems developed depend on alterations of metabolic and/or inflammatory pathways, whether by genetic changes, dietary interventions, or, more recently, drugs. The specific mutants, diets, or drugs chosen each year depend on scientific discoveries and interactions in each preceding year. Specific Aims: Aim 1 will support six externally funded projects (EPs) in year 1, including work on serotonin receptor mutants, drug effects on gene expression patterns, effects of anti- aging drugs on cancer cells, work on cap-independent translation of specific mRNAs, studies of adipose tissue inflammation, and an analysis of over-expression of an enzyme that protect mitochondria from oxidative damage. Aim 2 uses CFAR resources to maintain breeding colonies of special interest to mouse aging research, including mutants with tissue-specific alterations in Growth Hormone receptor, the IGF1 binding protein protease PAPPA, the mitochondrial redox protecting enzyme thioredoxin reductase 2, and two proteins that promote cap-independent translation of mRNA subsets. Aim 3 focuses on training, consultation, and professional education, so that CFAR can provide sophisticated perspectives on mouse aging models to PES and REC applications, and to UM students, fellows and faculty, and contribute to development of national standards for optimal use of rodents in biogerontology. Aim 4 involves selection of one or two new innovative mouse models for development in each year of the award. CFAR has, since its inception in 1989, provided both local and national leadership in the development of new mouse models for research on the control of aging and its links to late-life diseases, and hopes to maintain this role in the next award period.