Community Engagement

社区参与 摘要 国家翻译与发展资源中社区参与的目标 蛋白质组学(NRTDP)是公布在资源范围内开展的研究，并使科学家能够 这个国家以一种健壮和可重复性的方式进行自上而下的蛋白质组学(TDP)。我们了解到在那里 仍然是广泛采用自上而下、蛋白质形式分辨测量的障碍。穿过 社区参与，加上在此次更新中提出的研发5、6和7，我们的目标是分解 这些壁垒存在的地方，开发和输出的方法和技术将带来广泛的传播 采用TDP。 在最初的应用程序中，社区参与分为三个单独的部分：协作 和服务(CS)、培训和传播。我们认为这些区别仍然是有价值的，为此 续订时，我们保留这些部分中的每一个部分作为社区参与部分的具体目标。 具体目标1(协作和服务)。在这里，我们为NRTDP用户提供对TDP的直接访问，已执行 由我们在NRTDP内的科学家进行。我们在两个子目标中做到这一点，发展强大的服务线，专注于 靶向自上而下蛋白质组学和定量无标签自上而下蛋白质组学(TDQ)。 具体目标2(培训)。为了实现这一目标，我们在TDP中提供直接的实践教育。我们已经提出了两项建议 方法：每季度自上而下的蛋白质组学课程和我们的NRTDP学者交流计划。 具体目标3(传播)。最后，我们的目标是通过以下方式为自上而下的蛋白质组学提供持久的资源 NRTDP通过提供可在未来几年轻松访问和重复使用的工具来实现。我们将通过以下方式做到这一点 我们开发的软件以及发布健壮、标准化的方法和协议。

Community Engagement Abstract The goal of community engagement within the National Resource for Translational and Developmental Proteomics (NRTDP) is to promulgate the research developed within the Resource and enable scientists across the nation to perform top-down proteomics (TDP) in a robust and reproducible way. We understand that there are still impediments to the wide-scale adoption of top-down, proteoform-resolved measurements. Through Community Engagement, coupled with TR&D 5, 6 and 7, presented in this renewal, we aim to break down those barriers where they exist and develop and export the methods and techniques that will bring wide-spread adoption of TDP. In the initial application, Community Engagement was split into three separate sections: Collaboration and Service (CS), Training, and Dissemination. We feel that these distinctions are still valuable and for this renewal, we retain each of these sections as specific aims within the Community Engagement section. Specific Aim 1 (Collaboration and Service). Here we provide NRTDP users direct access to TDP, performed by our scientists within the NRTDP. We do so in two subaims, developing robust service lines focused on targeted top-down proteomics and quantitative label-free top-down proteomics (TDQ). Specific Aim 2 (Training). In this aim, we provide direct, hands-on education in TDP. We have proposed two methodologies: a quarterly, top-down proteomics course and our NRTDP Scholar Exchange Program. Specific Aim 3 (Dissemination). Finally, we aim to provide enduring resources for top-down proteomics through the NRTDP by providing tools that can be easily accessed and reused for years to come. We will do so through software that we develop as well as publishing robust, standardized methods and protocols.