COBRE Phase 2: Center for Neuroplasticity at the University of Puerto Rico

项目摘要 本申请的主要目标是购买四件新设备，以提高 UPR COBRE神经可塑性中心（UPR-CCfN）和神经影像学， 分子科学研究中心的电生理设施（NIEF，https：//www.nief-upr.com/） 中心（MSRC，（http：//cicim.upr.edu/）。UPR-CCfN是一项合作努力， 研究人员从医学科学校园和生物学系在里约彼德拉斯 校园它的目标是了解广泛的神经学问题，包括破译 控制行为的神经回路，神经和心理健康障碍的起源， 自闭症，创伤后应激障碍，痴呆症，阐明酒精神经适应的分子基础，成瘾， 和神经保护。这一补充申请是22个国家共同努力的结果。 科学家来改进我们的COBRE UPR-CcfN。对各种服务的需求增加 必须由NIEF提供的应用程序已经大大增加， 用户的增长以及科学的跨学科性质。我们建议购买 以下四个新的设备：（1）尼康Eclipse LV 100 N偏光显微镜，（2） Cytiva AKTA Purifier 100 FPLC系统（3）LI-COR Odyssey Western Blot系统和（4） QuantStudio 3 PRO-实时PRC系统。在仔细评估了各种商业广告后 根据市场上的消息来源，我们确定这四种仪器是UPR-CCfN的理想选择。 这些工具还将影响最近在UPR-CcfN雇用的新调查员名单， 以及新的赠款项目，包括生物学相关的研究，如艾滋病毒相关的 痴呆症研究，离子通道的脂质组学分析洗涤剂复合物，以改善 结晶，血吸虫病神经肽的鉴定， 抗缺血性中风的神经保护性营养补充剂和天然产物类似物 设计拟议的文书将促进多学科合作， 研究人员在CcfN和其他机构内外的波多黎各。另一 NIEF发展的一个重要方面是该设施将为研究人员提供的服务 不久将被招募，在普遍定期审议期间从事基础和生物医学相关项目。此外，委员会认为， 升级后的NIEF将优化教师、博士后研究员和研究生的专业知识， 对竞争性研究至关重要的技能。最后，这些工具将极大地影响 目前由NIH、NASA和NSF支持的大量学生群体的发展- 赞助的研究金或助学金。

Project Abstract The main goal of this application is to purchase four new pieces of equipment to enhance the UPR COBRE Center for Neuroplasticity (UPR-CCfN) and the Neuroimaging and Electrophysiological Facility (NIEF, https://www.nief-upr.com/) at the Molecular Science Research Center (MSRC, (http://cicim.upr.edu/). The UPR-CCfN is a collaborative effort between researchers from the Medical Sciences Campus and the Department of Biology at the Rio Piedras Campus. Its goal is to understand a wide range of neurological problems, including deciphering the neural circuits that control behavior, the origins of neural and mental health disorders such as autism, PTSD, dementia, elucidating the molecular basis for alcohol neuroadaptation, addiction, and neuroprotection. This supplemental application is the result of the collaborative efforts of 22 scientists to improve our COBRE UPR-CCfN. The increased demands on the variety of services and applications that must be provided by the NIEF have greatly increased based on its significant growth of users as well as the interdisciplinary nature of the science. We propose purchasing the following four new pieces of equipment: (1) Nikon Eclipse LV100N Polarized microscope, (2) Cytiva AKTA Purifier 100 FPLC System (3) LI-COR Odyssey Western Blot system and (4) QuantStudio 3 PRO-Real Time PRC system. After carefully evaluating various commercial sources on the market, we have determined these four instruments are ideal for the UPR-CCfN. These instruments will also impact a list of new investigators recently hired at the UPR-CCfN, as well as new grant projects that include biologically relevant studies such as HIV-associated dementia studies, lipidomic analysis of ion channels detergent-complexes to improve crystallization, schistosomiasis neuropeptide characterization, characterization of a neuroprotective nutritional supplement against ischemic stroke, and natural product analogs design. The proposed instruments will catalyze the multidisciplinary collaborations between researchers at the CCfN and other institutions within and outside of Puerto Rico. Another important aspect of the NIEF's development is the services the facility will provide to researchers being recruited shortly to pursue basic and biomedical-relevant projects at the UPR. Furthermore, the upgraded NIEF will optimize faculty, postdoctoral fellows, and graduate student expertise and skills so vital for competitive research. Finally, these instruments will greatly impact the development of the large student population currently supported by NIH, NASA, and NSF- sponsored fellowships or assistantships.