Discovery of Molecular Probes and Therapeutic Leads: Upgrade of an Electron Paramagnetic Resonance Spectrometer

摘要：购买布鲁克E500 CW需要行政补充 支持P20 GM 104316的电子顺磁共振（EPR）光谱仪： 化学探针和治疗先导物的发现。眼镜蛇的目标是 继续我们的努力，开发分子方法来探测生物学， 并应用新的化学生物学工具研究相关的生物学途径， 与疾病，并开发计算方法来了解小 分子与复杂大分子靶点的相互作用。这个目标 行政补充申请是为升级布鲁克E500 CW 具有X波段和Q波段的电子顺磁共振（EPR）谱仪 能力的升级将支持购买微波桥控制器，数字 超高分辨率霍尔场控制器和双通道信号处理单元 (SPU)和SuperX精选的超低噪声CW X波段微波电桥。的 光谱仪将与我们现有的磁铁，Q波段桥和我们现有的氦接口 VT-单元。升级将取代旧的EMX-EPR仪器， 功能超过10年。目前，没有一个有效的生产者延伸责任文书， UD校园EPR的可用性将支持当前的COBRE研究人员， 将是重要的招聘新的教师到UD校园在COBRE-related 研究领域。

ABSTRACT: An administrative supplement is requested to purchase Bruker E500 CW Electron Paramagnetic Resonance (EPR) spectrometer to support P20 GM104316: Discovery of Chemical Probes and Therapeutic Leads. The goal of this COBRE is to continue our efforts to develop molecular approaches for probing biology, to discover and apply new chemical biology tools for the study of biological pathways associated with disease, and to develop computational approaches for understanding small molecule interactions with complex macromolecular targets. The goal of this administrative supplement is to request funds for the upgrade of a Bruker E500 CW Electron Paramagnetic Resonance (EPR) spectrometer with both X-band and Q-band capabilities. The upgrade will support purchase of a Microwave Bridge Controller, Digital Ultra High Resolution Hall Field Controller, and Dual Channel Signal Processing Unit (SPU) and a SuperX selected ultra low noise CW X-band microwave bridge. The spectrometer will interface with our existing magnet, Q-band bridge and our existing He VT-unit. The upgrade will replace an old EMX-EPR instrument that has been non- functional for more than 10 years. Currently, there is no functioning EPR instrument on the UD campus. The availability of EPR will support current COBRE researchers and will be important for the recruitment of new faculty to the UD campus in COBRE-related research areas.