Core--Mouse Pathobiology

将建立老鼠病理生物学核心，以提供必要的设施以及教职员工的专业知识来支持所有三个研究项目。核心的一个主要目标将是提供在小鼠病理学方面的专业知识，以及对乳腺和乳腺癌病理学以及转移的病理生物学的最新熟练程度。病理学支持将由兽医解剖和临床病理学家提供，他们是美国兽医病理学家学会的外交官。在比较乳腺病理学方面的专业知识将有助于在研究项目中对小鼠模型进行适当的解释和评估。 并与人类乳腺癌进行比较。此外，一名小鼠病理学博士后实习生将在教职病理学家的监督下进行小鼠尸检(尸检)、组织切割和处理以及组织病理学评估。这将为研究项目提供卓越和持续的服务。调查人员还将提供其他服务，包括(1)小鼠临床病理学，包括配备全方位血液学和临床化学实验室的兽医教学医院，该实验室可适当分析包括小鼠在内的所有物种的样本；(2)在小鼠成像方面的专长，包括Faxitron高分辨率摄影、闪烁照相以及全动物或全动物器官生物发光和荧光成像；(3)支持转移研究，包括将肿瘤组织原位植入乳腺或肺内，将肿瘤细胞注入左心室以诱导骨转移；(4)支持在不同基因改变的小鼠之间进行乳腺移植。老鼠病理生物学核心学院将由兽医生物科学系组织。设施将包括四个尸检室(包括一个专门用于核心的验尸室)和一台自动达科免疫染色仪，显微检查设施包括一台可为会议输出视频的10头显微镜、专用生物发光和荧光活体成像仪器、数码大体和显微摄影设备、组织形态测量设备(包括荧光显微镜和BioQuant Nova分析软件)、小鼠手术和兽医学院的放射设备(包括高分辨率闪烁成像)。这些教师和服务将为研究项目提供独特的资源，并显著提高研究数据的质量和有效性。

The Mouse Pathobiology Core will be established to provide the necessary facilities and faculty and staff expertise to support all three research projects. A major goal of the Core will be to provide expertise in mouse pathology with up-to-date proficiency in mammary gland and breast cancer pathology and the pathobiology of metastasis. Pathology support will be provided by veterinary anatomic and clinical pathologists that are Diplomats of the American College of Veterinary Pathologists. Expertise in comparative mammary gland pathology will enable appropriate interpretation and evaluation of the mouse models in the research projects and comparison to human mammary cancer. In addition, a D.V.M. postdoctoral trainee in mouse pathology will perform the mouse necropsies (autopsies), cutting and processing of tissues and evaluation of histopathology under the supervision of faculty pathologists. This will provide excellence and continuity of service for the research projects. Other services that will be available to investigators include (1) mouse clinical pathology, including a full-service hematology and clinical chemistry laboratory in the Veterinary Medical Teaching Hospital that can appropriately analyze samples from all species including mice; (2) expertise in mouse imaging, including Faxitron high resolution radiography, scintigraphy, and whole animal or organ bioluminescent and fluorescence imaging; (3) support for studies on metastasis including orthotopic implantation of tumor tissue into the mammary glands or lungs and injection of tumor cells into the left cardiac ventricle to induce bone metastasis; and (4) support for transplantation of mammary gland anlage between mice with different genetic alterations. Faculty for the Mouse Pathobiology Core will be organized from the Department of Veterinary Biosciences. Facilities will include four necropsy rooms (including one devoted to the Core) and an automated Dako immunostainer, microcopy facilities including a 10-headed microscope with video output for conferences, dedicated bioluminescent and fluorescent in vivo imaging instrumentation, digital gross and microscopic photography facilities, histomorphometry equipment including a fluorescent microscope and Bioquant Nova analysis software, mouse surgery and radiology facilities including high resolution scintigraphy in the Colleges of Veterinary Medicine and Medicine. These faculty and services will provide a unique resource to the research projects and significantly enhance the quality and validity of the research data.