THE EFFECT OF GENISTEIN AND ISOFLAVONE METABOLITES ON CALCIUM ABSORPTION AND

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 几种类型的植物性产品含有芦荟酮正在销售作为替代雌激素替代疗法，以预防绝经后妇女的骨质疏松症。 雌激素抑制骨吸收，并可能增加钙的吸收。 对骨骼健康的疗效有喜忧参半的结果，这可能取决于剂量和相互作用的β-羟黄酮和个人的能力，以产生平等的。 本项目将确定染料木黄酮对雌马酚和非雌马酚生产者绝经后妇女钙吸收和骨吸收的剂量反应以及与其他大豆异黄酮的相互作用。 我们将使用41 Ca，一种长寿命的同位素，来标记骨骼和加速器质谱（AMS）来监测饮食的扰动。 这种研究骨吸收的创新方法是骨吸收的直接测量，与骨转换的生化标志物和传统的钙动力学相比，与研究骨密度变化相比快速，并且不像骨组织形态计量学那样具有侵入性。 我们能够通过测量尿液中41 Ca排泄的变化，系统地确定不同类型和剂量的含炔雌醇的植物产品在相同的绝经后妇女中顺序地抑制骨吸收的有效性的效果。 作为阳性对照，将比较阿托酮与Actonel（一种常见的骨质疏松症治疗方法）的效果。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Several types of botanical products containing isoflavones are being marketed as an alternative to estrogen replacement therapy to prevent osteoporosis in postmenopausal women. Estrogen suppresses bone resorption and may increase calcium absorption. Efficacy of isoflavones on bone health has mixed results which may depend on the dose and interaction of isoflavones and the ability of individuals to produce equal. This project will determine the dose response of effect of genistein and the interaction with other soy isoflavones on calcium absorption and bone resorption in postmenopausal women who are equol vs. nonequol producers. We will use 41Ca, a long-lived isotope, to label the skeleton and an Accelerator Mass Spectrometry (AMS) to monitor perturbations by diet. This innovative approach to study bone resorption is a direct measure of bone resorption in contrast to biochemical markers of bone turnover and traditional calcium kinetics, rapid compared to studying changes in bone density, and not as invasive as bone histomorphometry. We are able to systematically determine the effect of various types and dosages of isoflavone-containing botanical products sequentially in the same postmenopausal women for their effectiveness in suppressing bone resorption by measuring changes in 41Ca excretion in the urine. As a positive control the effect of isoflavones will be compared to Actonel , a common osteoporosis treatment therapy.