UNMC: MICROARRAY CORE, GENOMICS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在INBRE赠款的第一年，微阵列核心设施向INBRE的同事以及内布拉斯加州的其他学术研究人员提供了服务和支持。资金用于购买试剂，以制作和处理载玻片，升级对设施运营至关重要的微阵列专用仪器，并提供人员支助，包括为一名研究技术员提供50%全时当量，为核心主任提供15%全时当量。INBRE项目获得的支持对核心设施向学术界提供功能基因组学支持的能力产生了巨大影响。下文概述了自2004年6月以来核心工作取得的成就。 微阵列服务和支持第四年 INBRE Associates： 内布拉斯加州大学科尔尼分校的金·卡尔森博士：该机构已经与卡尔森博士完成了一系列微阵列实验(分析正在进行中)，以使用果蝇Affymetrix GeneChips进行功能基因组学实验。这个具体的项目是以果蝇为模型系统，确定OTK18过度表达对发育和免疫基因的基因调控。 道格·克里斯滕森博士-韦恩州立大学：该设施已经打印了一个定制的斑点微阵列来调查单核细胞增多性李斯特菌的基因表达。基因表达实验即将开始。这些数据将被用来进一步了解与李斯特菌相关的毒力因子，以及人类免疫系统的细胞如何可能或可能不会刺激这种病原体的独特基因转录。 Barbara Clement Phd-Doane学院：核心设施一直在与Clement博士合作设计实验，研究膜通透性对铜绿假单胞菌检测群体感应(QS)信号(N-酰基高丝氨酸内酯或AHL)能力的影响。实验正在进行中。 Garry Duncan博士，内布拉斯加州卫斯理大学：该机构一直在与Duncan博士合作，设计一种定制的斑点微阵列，以调查草履虫Bursaria小球藻病毒-1(PBCV-1)的基因表达。初步打印和随后的基因表达数据很耐人寻味。这种病毒是一种古老的病毒，具有一些非典型的分子机制，因此可以深入了解新陈代谢和其他生物过程的进化。 凯瑟琳·塔尔曼博士多恩学院：该机构一直在与塔尔曼博士合作设计实验，研究酒精暴露后小鼠基因表达的变化。这旨在为胎儿酒精综合征的研究提供一个模型。 非INBRE调查人员： 斑点阵列平台：CORE使用Brin基金购买的寡核苷酸集和机器人Spotter，例行打印和处理代表人类、小鼠和大鼠基因组的10K幻灯片集。Core还为研究人员打印定制阵列。除上述INBRE用户外，该机构还为来自内布拉斯加州四个机构的20名调查人员提供服务。这些研究人员中有15人在UNMC，3人在UNL，1人在UNO，1人在克雷顿大学。 Affymetrix平台：Brin支持实验室的研究技术员为位于内布拉斯加州三个机构的21名调查人员处理Affymetrix芯片。其中包括UNMC的19名调查人员、UNO的1名调查人员和博伊斯敦国家研究医院的1名调查人员。 以上调查人员的研究领域实例：视网膜疾病、前列腺癌、淋巴瘤、帕金森？S病、甲基化模式与癌症、艾滋病毒相关神经变性、细菌感染相关疾病、白血病、肺部疾病、发育性心脏缺陷、心力衰竭、青光眼、口腔癌、听力损失遗传学、脑垂体瘤、乳腺癌、糖尿病。 设备采购 第四年的资金被用来为机器人观察者购买了32个新的打印机针脚。这是保持观察者打印载玻片斑点均匀所必需的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Microarray Core Facility has provided service and support to INBRE associates as well as other academic investigators in the State of Nebraska during Year #1 of the INBRE grant. Funds were used to purchase reagents to fabricate and process slides, upgrade microarray specific instrumentation critical to the operations of the facility, and to provide personnel support including 50 % FTE for a research technologist and 15% FTE for the director of the core. The support received from the INBRE program has had a tremendous impact on the ability of the core facility to provide functional genomics support to the academic community. Outlined below are the accomplishments of the core since June of 2004. MICROARRAY SERVICES AND SUPPORT YEAR #4 INBRE Associates: Kim Carlson Ph.D. University of Nebraska at Kearney: The facility has completed a set of microarray experiments (analysis underway) with Dr. Carlson to perform a functional genomics experiment using Drosophila Affymetrix GeneChips. The specific project is determining the gene regulation of developmental and immune genes as a result of OTK18 overexpression using Drosophila as a model system. Doug Christensen PhD-Wayne State: The facility has printed a custom spotted microarray to survey Listeria monocytogenes gene expression. Gene expression experiments will begin soon. This data will be used to further knowledge of virulence factors associated with Listeria and how cells of the human immune system may or may not stimulate unique gene transcription in this pathogen. Barbara Clement PhD-Doane College: The core facility has been working with Dr. Clement designing experiments to study the effect of membrane permeability on the ability of Pseudomonas aeruginosa to detect quorum sensing (QS) signals (N-acyl homoserine lactones or AHL). Experiments are underway. Garry Duncan PhD Nebraska Wesleyan University: The facility has been working with Dr. Duncan designing a custom spotted microarray to survey Paramecium bursaria Chlorella virus-1 (PBCV-1) gene expression. Preliminary printing and subsequent gene expression data is intriguing. This virus is an ancient virus with some atypical molecular mechanisms and thus may yield insight into evolution of metabolism and other biological processes. Kathleen Tallman PhD-Doane College: The facility has been working with Dr. Tallman designing experiments to study changes in gene expression in mice following ethanol exposure. This is intended to serve as a model for fetal alcohol syndrome studies. Non-INBRE Investigators: Spotted Array Platform: The core routinely prints and processes 10K slide sets representing human, mouse and rat genomes using the oligonucleotide sets and robotic spotter purchased with BRIN funds. The Core also prints custom arrays for researchers. In addition to the INBRE users listed above, the facility serviced 20 investigators from four institutions in the State of Nebraska. 15 of these researchers were at UNMC, 3 were at UNL, 1 at UNO, and 1 at Creighton University. Affymetrix Platform: The BRIN supported research technologist in the laboratory processed Affymetrix chips for 21 investigators located at three institutions in Nebraska. These include 19 investigators at UNMC, 1 at UNO, and 1 at Boystown National Research Hosptital. Examples of Research Areas Of Above Investigators: Retinal disease, prostate cancer, lymphoma, Parkinson?s disease, methlyation patterns and cancer, HIV associated neural degeneration, bacterial infection associated disease, leukemia, pulmonary disease, developmental heart defects, heart failure, glaucoma, oral cancer, genetics of hearing loss, pituitary tumors, breast cancer, and diabetes. EQUIPMENT PURCHASES Funds from year #4 were used to purchase 32 new printer pins for the robotic spotter. This is necessary to keep the spotter printing slides with uniform spots.