FLOW CYTOMETRY CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 流式细胞仪共享资源为研究人员提供了对流式细胞仪和细胞分类的访问。细胞分析共享资源的目标是为所有感兴趣的研究人员提供全面的数据收集、数据分析、数据解释和流式细胞术方面的教育。这包括适当的样品准备以及对流式细胞术的理论和实践的教育。流式细胞仪共享资源提供了建立和处理用于流动分析的标本的基本指导。这些包括：免疫荧光(1-15种颜色，1-17个参数)；标准CD标记的表面表达；定制抗体的表面标记的专门表达；基因转导/表达；荧光蛋白；受体表达；细胞凋亡；Annexin V结合；caspase活性；DNA分析；细胞周期分析；细胞凋亡；癌症治疗；动力学研究(时间依赖性分析)；细胞分选；细胞浓缩；细胞纯化；单细胞克隆；新的方案开发；以及将新技术引入流式细胞术共享资源现有服务曲目的初步研究。流式细胞仪共享资源利用最新版本的分析软件提供对流式细胞仪数据的数据分析。在本报告所述期间，该机构为林肯校区的18名调查人员和内布拉斯加大学医学中心(奥马哈)校区的76名调查人员提供服务。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Flow Cytometry Shared Resource provides researcher access to flow cytometry and cell sorting. The goal of the Cell Analysis Shared Resource is to provide comprehensive data collection, data analysis, data interpretation and education in flow cytometry to all interested investigators. This includes proper sample preparation as well as education in theory and practice of flow cytometry. The Flow Cytometry Shared Resource provides basic direction on the set up and processing of specimens for flow analysis. These include: immunofluorescence (1-15 colors, 1-17 parameters); surface expression of standard CD markers; specialized expression of surface markers with custom antibodies; gene transduction/expression; fluorescent proteins; receptor expression; apoptosis; Annexin V binding; caspase activity; DNA analysis; cell cycle assays; apoptosis; cancer therapy; kinetic studies (time dependent assay); cell sorting; cell enrichment; cell purification; single cell cloning; new protocol development; and preliminary studies to bring new techniques into the repertoire of available services of the Flow Cytometry Shared Resource. The Flow Cytometry Shared Resource provides data analysis of flow cytometric data utilizing the most current versions of analysis software.. During the period covered by this report, the facility served 18 investigators on the Lincoln Campus and 76 investigators on the University of Nebraska Medical Center (Omaha) campus.