CRYSTAL STRUCTURE OF THE RIBOSOME

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们的目标是（a）获得70 S核糖体晶体的更高分辨率，目前限制为5.0，和（B）获得额外的核糖体功能复合物的晶体结构，包含各种功能状态的mRNA，tRNA和翻译因子。 我们首先计划筛选我们最近获得的几十种70 S核糖体和核糖体功能复合物的晶体，包括至少两种不同晶体形式的晶体。 如果我们在筛选期间观察到有希望的衍射结果，我们将根据可用时间收集数据。 由于我们的单胞尺寸很大，衍射很弱，所以有必要在同步加速器上进行筛选。 出于同样的原因，我们更喜欢使用高通量的光束线并使用大型探测器，以获得足够的强度和斑点间隔。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our goals are to (a) obtain improved resolution of crystals of 70S ribosomes, currently limited to 5.0 ¿, and (b) to obtain crystal structures of additional ribosome functional complexes, containing mRNA, tRNAs and translational factors in various functional states. We first plan to screen several dozen crystals of 70S ribosomes and ribosome functional complexes that we have recently obtained, including ones from at least two distinct crystal forms. If we observe promising diffraction results during screening, we will collect data, according to the time available. It is necessary for us to carry out screening at the synchrotron because of the very large size of our unit cells, and consequent weak diffraction. For the same reason, we prefer to use beamlines with high flux, and using large detectors, to obtain adequate intensities and spot separation.