SAXS STUDIES OF METALLOPROTEINS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 (1)MtmB-MtmC复合物-我们能够收集关于MtmB复合物的数据，所述MtmB复合物由与六个MtmC单元（29 kDa）结合的MtmB亚基（6 x 60 kDa）的六聚体组成。他们能够得到合理的数据复杂。我们希望通过单独收集MtmB六聚体的数据来完成这项研究，以便我们可以更好地识别结构差异。我们有MtmB六聚体和MtmC的晶体结构，并试图将数据与MS研究相关联。另一个值得注意的特征是MtmB含有第22个遗传编码的氨基酸吡咯赖氨酸，其用于激活底物转移到MtmC。(2)嗜热栖热菌羧肽酶-我们在JBC有一篇关于这种蛋白质晶体结构的论文正在修订中。作为修订的一部分，他们要求我们验证其在溶液中的二聚化。在相关系统上的凝胶过滤给出83 kDa的异常结果，与单体59 kDa和二聚体118 kDa相比。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. (1) MtmB-MtmC complex - We were able to collect data on the MtmB complex comprised of a hexamer of MtmB subunits (6 x 60 kDa) bound to six MtmC units (29 kDa). They were able to get reasonable data for the complex. We would like to complete this study by collecting data on the MtmB hexamer alone so that we can better identify the structural differences. We have crystal structures of the MtmB hexamer and MtmC and are trying to correlate the data with MS studies also ongoing. Another notable feature is that MtmB contains the 22nd genetically encoded amino acid pyrrolysine which is used to activate substrate transfer to MtmC. (2) Thermus thermophilus carboxypeptidase -We have a paper in revision at JBC on the crystal structure of this protein. As part of the revision, they have asked us to verify its dimerization in solution. Gel filtration on related systems give an anomalous result of 83 kDa compared to the monomer 59 kDa and dimer 118 kDa.