COBRE: UNV MED SCH: CORE C: CELL PROTEOMICS INTERFACE FACILITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 Cobre需要人员与位于UNR的内华达州两个核心设施--蛋白质组学和生物信息学--对接，以增强我们完成与蛋白质组学方法相关的目标的能力。内华达州的两个核心没有足够的工作人员来完全满足科布雷的需求。核心将有助于减轻私营部门的工作量，使他们能够专注于其项目的主要目标，同时探索新的方向，这将导致新的问题。核心运营概述。核心将作为5个项目的技术支持。该核心的主要功能如下。1)与核心总监和每个私人助理合作，为每个项目的蛋白质组学部分制定详细的实验方法。2)促进从组织或培养的细胞中制备适合问题的可重复的亚细胞部分。3)与内华达州蛋白质组学核心设施合作，使用适当的技术来识别、确定这些亚细胞组分中蛋白质的相对数量和翻译后修饰。4)与内华达州蛋白质组学核心设施合作，协助进行质谱学数据分析。5)与内华达生物信息学核心合作，协助计算方法来注释和识别新发现的蛋白质的功能。6)与内华达州生物信息学核心合作，协助如何将获得的信息纳入数据库，并提供进入生物医学界的途径。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The COBRE needs personnel to interface with two Nevada core facilities here at UNR, Proteomics and Bioinformatics, to enhance our ability to complete aims related to proteomics approaches. The two Nevada cores do not have sufficient staff to fully meet the needs for the COBRE. The core will serve to relieve the workload on the P. I.s by allowing them to focus upon the main aims of their projects and at the same time explore new directions which will lead to new questions. Overview of Core Operations. The core will serve as technical support for the 5 projects. The main functions of the core are as follows. 1) Work with the Core Director and each P. I. to develop a detailed experimental approach for proteomics component of each project. 2) Facilitate the preparation of reproducible subcellular fractions from tissues or cultured cells that are appropriate to the question. 3) Use appropriate techniques to identify, determine relative amounts, and post-translational modifications of proteins in these subcellular fractions, in collaboration with the Nevada Proteomics Core Facility. 4) Assistance with mass spectral data analysis in collaboration with the Nevada Proteomics Core Facility. 5) Assistance with computational methods to annotate and identify function of newly discovered proteins, in collaboration with the Nevada Bioinformatics Core. 6) Assistance with how to incorporate the information obtained into the database and provide access to the biomedical community, in collaboration with the Nevada Bioinformatics Core.