MOLECULAR CORE

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该核心将重点支持COBRE资助的干细胞研究的分子方面，如基因表达和功能。 通过真实的时间RT-PCR（ABI 7000序列检测系统）在细胞周期的各个阶段观察细胞表型和功能。 将支持载体构建、细胞转染和转染细胞的表型分析。 将根据需要使用其他分子检测方法。 核心设备包括真实的实时RT-PCR序列检测系统（Applied Biosystems）、GenePCR系统2700（Applied Biosystems）和BioRad GelDoc XR（带UV透照仪）。 核心提供的服务将包括关于真实的时间PCR机器和载体构建的培训。 COBRE参与者可以使用该核心，不会向用户收取任何费用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This core will focus on supporting the Molecular aspects of COBRE funded stem cell studies such as gene expression and function. Cell phenotype and function will be observed by real time RT-PCR (ABI 7000 Sequence Detection System) at various stages in cell cycle. Vector construction, cell transfection and phenotyping of transfected cells will be supported. Other molecular methods of detection will be used as needed. The core equipment consists of a real time RT-PCR Sequence Detection System (Applied Biosystems), GeneAmp PCR System 2700 (Applied Biosystems) and a BioRad GelDoc XR with UV Transilluminator. Services provided by the core will include training on the Real Time PCR machine and vector construction. The core is available to COBRE participants and no fees will be charged to users.