FLOW CYTOMETRY CORE

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 流式细胞仪共享资源为研究人员提供流式细胞仪和细胞分选的访问。细胞分析共享资源的目标是为所有感兴趣的研究人员提供全面的数据收集，数据分析，数据解释和流式细胞术教育。这包括适当的样品制备以及流式细胞术的理论和实践教育。流式细胞术共享资源提供了关于流式细胞术分析标本的设置和处理的基本指导。其中包括：免疫荧光（1-15种颜色，1-17个参数）;标准CD标记物的表面表达;使用定制抗体的表面标记物的专门表达;基因转导/表达;荧光蛋白;受体表达;细胞凋亡;膜联蛋白V结合;半胱天冬酶活性; DNA分析;细胞周期测定;细胞凋亡;癌症治疗;动力学研究流式细胞仪的主要功能包括：流式细胞术（时间依赖性测定）;细胞分选;细胞富集;细胞纯化;单细胞克隆;新方案开发;以及将新技术引入流式细胞术共享资源的可用服务库的初步研究。流式细胞术共享资源利用最新版本的分析软件提供流式细胞术数据的数据分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Flow Cytometry Shared Resource provides researcher access to flow cytometry and cell sorting. The goal of the Cell Analysis Shared Resource is to provide comprehensive data collection, data analysis, data interpretation and education in flow cytometry to all interested investigators. This includes proper sample preparation as well as education in theory and practice of flow cytometry. The Flow Cytometry Shared Resource provides basic direction on the set up and processing of specimens for flow analysis. These include: immunofluorescence (1-15 colors, 1-17 parameters); surface expression of standard CD markers; specialized expression of surface markers with custom antibodies; gene transduction/expression; fluorescent proteins; receptor expression; apoptosis; Annexin V binding; caspase activity; DNA analysis; cell cycle assays; apoptosis; cancer therapy; kinetic studies (time dependent assay); cell sorting; cell enrichment; cell purification; single cell cloning; new protocol development; and preliminary studies to bring new techniques into the repertoire of available services of the Flow Cytometry Shared Resource. The Flow Cytometry Shared Resource provides data analysis of flow cytometric data utilizing the most current versions of analysis software.