Imaging Core

我们最近收到资金，将过时的BioRad 600共焦显微镜更换为徕卡TCS SP 光谱共聚焦显微镜(S10 RR16708)。新的共焦的日常运行是在 爱德华·多尔蒂先生，一位经验丰富的显微镜专家。多尔蒂的工资目前部分由果蝇支付 计划项目赠款(44232新台币)，部分由马德N S 17813提供，部分由生物系资金提供。这个 Brandeis的所有调查人员都可以按使用费使用ConFocus。目前，共焦系统的使用率很高 (60-70小时/周)，因为多个实验室(Marder、White、Hall、Griffith)的项目高度依赖 共焦，用于研究固定的和处理过的组织。 迫切需要更多的仪器和人员来满足我们用户群体的需要。一个 我们用户组的子集(Turrigiano[PI]、Marder、Griffith、Lisman和Nelson)申请共享设备 授予购买徕卡光谱共焦/多光子显微镜，以允许研究活组织梳理 电生理学(S10 RR017938)。这笔赠款做得很好，有很好的机会获得资助。 该大学已承诺提供资金安装该仪器并进行翻新以适应它，但 目前没有机构资金可供支助人员使用。Ed Dougherty先生将负责监督 新的共焦和多光子仪器，但这些新设备的日常运行将 需要额外的技术人员。我们也缺乏资金来购买完全需要的电生理设备 利用多光子装置的潜力。电生理学与多光子相结合的可行性 对用户群体进行成像将使我们能够在重要的新方向上扩展我们的研究计划。 我们成像武器库的第二个缺口是缺乏用于延时成像的仪器和软件。一个 我们用户组的许多项目需要对多个荧光团进行时间推移测量。设置成本 对于这种成像设备(操纵器、刺激器、培养室舞台)来说，任何人都不能接受，因为 它不会在一个实验室里得到全天候的使用。为这类分析增加设备和软件 将扩展现有项目，并允许用户调查这些新的、高度相关的研究途径。

We recently received funding to replace an obsolete BioRad 600 confocai microscope with a Leica TCS SP Spectral confocal microscope (S10 RR16708). The day-to-day running of the new confocal is under the direction of Mr. Edward Dougherty, an experienced microscopist. Dougherty's salary is currently paid in part by the Drosophila program project grant (N $44232), partly by Marder's N S 17813, and partly by Biology Department funds. The confocal is available on a fee-for-usage basis for all investigators at Brandeis. The confocal is presently heavily used (60-70 hours/week) as several laboratories (Marder, White, Hall, Griffith) have projects that intensively depend on the confocal, for the study of fixed and processed tissue. There is an urgent need for additional instrumentation and personnel to meet the needs of our user group. A subset of our user" group (Turrigiano [PI], Marder, Griffith, Lisman and Nelson) applied for a Shared Equipment Grant to purchase a Leica Spectral Confocal/Multiphoton microscope, to allow for the study of live tissue combhled with electrophysiology (S10 RR017938). This grant did very well and stands an excellent chance of being funded. The University has committed funds for the installation of this instrument and renovations to accommodate it, but there are currently no institutional funds available for support personnel. Mr. Ed Dougherty will provide oversight for the new confocal and the multiphoton instrument, but the day-to-day running of these new pieces of equipment will require an additional technician. We also lack funds to purchase the electrophysiology equipment needed to fully utilize the potential of the multiphoton set-up. The availability of combined electrophysiology and multiphoton imaging to the user group would allow us to extend our research programs in important new directions. A second gap in our imaging arsenal is the lack of instrumentation and software for time-lapse imaging. A number of the projects of our user group require time-lapse measurements of multiple fluorophores. The set-up costs for this imaging rig (manipulators, stimulators, culture chamber stage) have been prohibitive for any individual, since it would not receive full-time use in a single lab. The addition of equipment and software for this type of analysis would extend existing projects and allow users to investigate these new, highly relevant avenues of research.