Network for Premature Birth Research Analytical Core

该提案旨在创建一个分析核心，该核心将服务于基因组学和蛋白质组学 早产研究网络。分析核心将有五个组成部分，将满足 需要1）样本处理、储存和分发/检索; 2）高通量基因分型， 基因研究的解释; 3）微阵列分析和数据挖掘; 4）蛋白质组学，脂质组学和 氧化应激的评估;和5）生物信息学和统计学。该核心的每个组成部分将 由具有特定方法学和数据解释专门知识的既定调查员监督。 主要研究者负责分析核心1职能的总体协调 他还将担任样本处理、储存、分发/检索部门的主任， 行政单位（杰罗姆F.施特劳斯，三世，医学博士，Ph.D.），与组件设施协同工作 基因分型和遗传分析：Richard S. Spielman博士;微阵列：Don Baldwin博士; 蛋白质组学/脂质组学和氧化应激：Ian Blair博士：生物信息学和统计学：大卫 Fenstermacher博士）他将担任分析核心指导委员会。校长 研究者将担任指导委员会主席，并与其他研究者进行沟通， NICHD参与该计划的人员。拟议的核心方案建立在联合国 宾夕法尼亚大学研究早产遗传学，复杂性状的遗传学， 蛋白质组学、氧化应激生物标志物研究和微阵列分析。现有设施包括 制备以适应高通量测定。此外，核心关键人员可以提供专家 研究设计和数据分析方面的建议。围绕混合映射的使用构建的概念协议 以及传递不平衡检验，以确定血统对早产和新生儿的影响 早产后的结果以及相关和联系研究，以确定特定的风险促进 保护性基因和亲本起源效应。

This proposal is submitted to create an Analytical Core, which will serve the Genomic and Proteomics Network for Premature Birth Research. The Analytical Core will have five components that will meet the needs for 1) sample processing, storage and distribution/retrieval; 2) high-throughput genotyping and interpretation of genetic studies; 3) microarray analysis and data mining; 4) proteomics, lipidomics and assessment of oxidative stress; and 5) Bioinformatics and statistics. Each component of this Core will be supervised by an established investigator with expertise in the specific methodology and data interpretation. The overall coordination of Analytical Core1 functions will be the responsibility of the Principal Investigator who will also serve as the Director of the sample processing, storage, distribution/retrieval component and administrative unit (Jerome F. Strauss, III, M.D., Ph.D.), working in concert with the component facility directors (Genotyping and genetic analysis: Richard S. Spielman, Ph.D.; Microarray: Don Baldwin Ph.D.; Proteomics/lipidomics and oxidative stress: Ian Blair, Ph.D.: Bioinformatics and statistics: David Fenstermacher, Ph.D.) who will serve as the Analytical Core Steering Committee. The Principal Investigator will serve as the Steering Committee Chair and the interface with the other investigators and N.I.C.H.D. staff participating in this program. The proposed Core builds on established strengths of the University of Pennsylvania in the study of the genetics of preterm birth, the genetics of complex traits, proteomics, the study of oxidative stress biomarkers, and microarray analysis. The existing facilities are prepared to accommodate high-throughput assays. In addition, the Core key personnel can provide expert advice in study design and data analysis. A Concept Protocol built around the use of admixture mapping and the transmission disequilibrium test to identify the impact of ancestry on preterm birth and neonatal outcomes after preterm delivery as well as association and linkage studies to identify specific risk promoting and protective genes, and parent of origin effects.