Proteomics

纽约大学癌症研究所蛋白质组学核心设施提供最先进的生物质谱分析 纽约大学癌症研究人员的分析，以帮助他们识别和表征医学蛋白质。 重要性该设施已经从SDS-PAGE凝胶中鉴定了数万种蛋白质，通常在 亚皮摩尔水平，通过蛋白质的LC-MS/MS，随后通过MASCOT或BLAST同源性数据库 寻找该设施还表征了翻译后修饰，如磷酸化， 酰化和糖基化。除了技术专长外，该设施的优势之一是其 在实验设计和解释方面为客户提供建议的能力，以便获得有用的数据 从这些数据中可以得到有意义的信息。依靠尖端技术及其专家 和专职工作人员，使这些服务能够以具有成本效益的方式进行。事实上许多 纽约大学和纽约大学癌症研究所的研究人员进行的实验如果没有 设施的帮助。最近，该机构建立了一个临床蛋白质组学核心，用于检测 用于癌症早期检测的蛋白质和肽生物标志物。

The NYU Cancer Institute Proteomics Core Facility provides state-of the-art biological mass spectrometry analyses to cancer researchers at NYU to help them identify and characterize proteins of medical importance. The facility has identified many tens of thousands of proteins from SDS-PAGE gels, often at subpicomolar levels, by LC-MS/MS of proteins followed by MASCOT or BLAST homology database searching. The facility has also characterized posttranslational modifications such as phosphorylation, acylation, and glycosylation. In addition to its technical expertise, one of the strengths of the facility is its ability to advise clients in the design and interpretation of experiments so that useful data can be obtained and meaningful information can be had from these data. Reliance on cutting edge technology and its expert and dedicated staff allow these services to be performed in a cost effective manner. Indeed, many experiments by investigators at NYU and the NYU Cancer Institute could not have been done without the assistance of the facility. Recently the Facility has established a Clinical Proteomics Core for the detection of protein and peptide biomarkers for the early detection of cancer.