DEVELOPMENT OF REVERSIBLE INACTIVATION TECHNIQUE STUDY HIGHER COGNITIVE FUNCTION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在我们的研究中，我们使用了最先进的可逆失活技术来更详细地探索猴子记忆功能中内侧颞叶不同组成部分之间的相互作用。这些方法允许在测试某些特定的记忆功能时，有选择地在短时间内关闭和打开内侧颞叶内的个别结构。这些可逆的失活过程创造了“虚拟损伤”，因为潜在的神经电路不会在结构上受到干扰，而特定的部位只是暂时失活。今年，一只成年雄性猴子接受了手术前的脑部核磁共振扫描，以确定我们的目标结构(海马体)的坐标位置。然后，手术植入位于每个海马体前部和后部并固定在骨上的两个引导管。这种动物目前正在接受行为训练，以测量空间记忆能力。在一半的测试过程中，目标结构被蝇草酚(GABA激动剂)灭活，而在另一半的测试过程中，则注射赋形剂。因此，每一种动物都被用作自己的对照。初步数据表明，蝇油在25分钟至1小时内达到最大行为效果，之后效果开始减弱。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In our studies we used state-of-the-art reversible inactivation techniques to probe in greater detail the interactions between different components of the medial temporal lobe in memory functions in monkeys. These methods permit individual structures within the medial temporal lobe to be selectively turned "off" and "on" for a short period of time while some specific memory functions are tested. These reversible inactivation procedures create "virtual lesions" as the underlying neural circuitry is not structurally disrupted and a particular site is only temporarily deactivated. In the current year, one adult male monkey received a presurgical MRI scan of the brain to determine the coordinate sites for our targeted structures (the hippocampi). Then, surgical implantation of two guide cannulae positioned at the anterior and posterior portions of each hippocampus and secured on the bone. This animal is currently behaviorally trained to measure spatial memory abilities. In half of the testing sessions the target structure is inactivated with muscimol (GABA agonist), and with the other half, vehicle is injected. Therefore each animal is used as its own control. The preliminary data indicate that muscimol achieved maximal behavioral effect between 25 minutes to one hour, after which the effects begin to wear off.