PROMOTER-BASED FUNCTIONAL MAPPING OF AMYGDALA MICROCIRCUITS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该项目使用细胞类型特异性启动子来驱动荧光报告子，以帮助从啮齿动物和灵长类动物杏仁核的器官型切片培养物内的特定神经元亚群进行光学引导的电生理记录。 在报告期间，我们继续研究胆囊收缩素（CCK）含有中间神经元的生理和形态学特性，使用CCK启动子标记的绿色荧光蛋白（GFP）。 然后将该慢病毒构建体用于体内转染神经元，仅那些正常表达CCK的神经元共表达GFP。 我们用这种方法来研究大鼠基底外侧杏仁核CCK中间神经元的生理特性。在报告期间，我们继续开发用于皮质中间神经元的小清蛋白、生长激素抑制素和钙视网膜蛋白亚群的慢病毒载体。 此外，我们致力于将光激活通道视紫红质（ChR 2）蛋白纳入我们的慢病毒骨架。 我们的技术将继续使用光来选择性地激活清醒行为动物的神经元亚群。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project uses cell-type specific promoters to drive fluorescent reporters to aid in optically guided electrophysiological recording from specific subpopulations of neurons within organotypic slice cultures of the rodent and primate amygdala. During the reporting period, we continued to investigate the physiological and morphological properties of cholecystokinin (CCK) containing interneurons using the CCK promoter tagged with a green fluorescent protein (GFP). This lentiviral construct was then used to transfect neurons in vivo, only those neurons that normally express CCK co-expressed GFP. We used this approach to examine the physiological properties of CCK interneurons in the rat basolateral amygdala. During the reporting period, we continued to develop lentiviral vectors for the parvalbumin, somatostatin, and calretinin subpopulations of cortical interneurons. In addition, we worked on incorporating light-activated channel-rhodopsin (ChR2) protein into our lentiviral backbone. Our technologies will continue to use light to selectively activate subpopulations of neurons in awake behaving animals.