800 MHz Low Temperature Magic Angle Spinning NMR Probe.

描述(由申请人提供)：申请资金支持购买低温魔角旋转(LT-MAS)探头，该探头将与800/89固态光谱仪一起使用。该探头包括用于冷却的低温平台、传输线和探头本身。LT-MAS探测器在90-300K的温度范围内工作，将用于检测低温保护的膜蛋白和淀粉样蛋白样品中的各种温度依赖现象。该探测器具有真空杜瓦、样品弹出能力，并包括一个用于未来527 GHz低温动态核极化(DNP)实验的波导。研究项目包括下列膜和淀粉样蛋白：(1)细菌视紫红质(BR)，(2)脂双层中的电压依赖性阴离子通道(VDAC)，(3)流感病毒的M2蛋白，(4)形成蛋白膜的气泡，以及由(5)磷脂酰肌醇-3-激酶SH3结构域(PI3-SH3)形成的淀粉样纤维，(6)与透析相关的淀粉样变性相关的β-2-微球蛋白，以及(7)酵母普恩系统的Sup35p蛋白。800/89上的LT-MAS探测器将是一个仪器集群的一部分，该仪器集群服务于波士顿地区执行高场生物核磁共振的约75名科学家社区。该仪器将缓解低温MAS实验对额外能力的迫切需求。此外，该仪器将对研究产生重大影响，因为它将采用一些普遍不存在的创新技术。此外，LT-MAS探头与800/89的组合将是北美为数不多的宽带式低温固态核磁共振仪器之一，因此将填补结构生物学的一个重要空白。

DESCRIPTION (provided by applicant): Funding is requested to support the purchase of a low temperature magic angle spinning (LT-MAS) probe that will be used with a 800/89 solid state spectrometer. The probe includes a cryogenic platform for cooling, transfer lines and the probe itself. The LT-MAS probe operates over the temperature range 90-300 K and will be used to examine a variety of temperature dependent phenomena in cryoprotected membrane protein and amyloid protein samples. The probe has a vacuum dewar, sample eject capabilities, and includes a waveguide for future low temperature dynamic nuclear polarization (DNP) experiments at 527 GHz. Research projects include the following membrane and amyloid proteins: (1) bacteriorhodopsin (bR), (2) the voltage dependent anion channel (VDAC) in lipid bilayers, (3) the M2 protein from influenza virus, (4) gas vesicles which form a proteinaceous membrane, and amyloid fibrils formed from (5) phosphatidyl inositol-3-kinase SH3 domain (PI3-SH3), (6) beta-2-microglobulin associated with dialysis related amyloidosis, and (7) the Sup35p protein, a yeast prion system. The LT-MAS probe on the 800/89 will be part of a cluster of instrumentation that serves a community of ~75 scientist performing high field biological NMR in the Boston area. The instrumentation will alleviate a pressing need for additional capabilities for low temperature MAS experiments. Further, the instrument will have a significant impact on research since it will employ a number of innovative technologies that are not generally available. In addition, the combination of the LT-MAS probe with the 800/89 will be one of the few widebore, low temperature solid state NMR instruments available in North America and will therefore fill an important void in structural biology.