DEVELOPED A VIEWER FOR CHIP-SEQ DATA

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 研制了一种在染色体上显示芯片序列数据的观察器。查看器使用户既可以获得对基因组的观察概览，也可以获得感兴趣区域的详细视图，在这些区域中，CHIP-SEQ数据与染色体注释一起显示，其中带有指向基因信息的超链接。查看器是基于网络的，以支持协作。数据被提交到队列进行处理，并且所有页面都被预先生成以允许在查看期间快速访问。该查看器最初用于分析组蛋白在不同修饰状态下的结合位置，并发表了一篇关于这项工作的论文(Siegel TN，Hekstra DR，Kemp le，Figueiredo LM，Lowell Je，Fenyo D，Wang X，Dewell S，Cross GA)。四种组蛋白变体标记了布氏锥虫多顺反子转录单位的边界。吉尼斯·德夫(Gene Dev.)2009年5月1日；23(9)：1063-76)。后来，查看器还被用来研究酿酒酵母中高表达基因的复制停滞。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A viewer to display ChIP-Seq data on chromosomes was developed. The viewer allows the user to both get an overview of the observations over the genome, and a detailed view of regions of interest where the ChIP-Seq data is shown with chromosomal annotations with hyperlinks to information about the genes. The viewer is web-based to support collaborations. The data is submitted to a queue for processing and all pages are pregenerated to allow fast access during viewing. The viewer was initially used to analyze the locations for binding of histones in different modification states in Trypanosoma bruzei (George Cross Lab) and a paper on this work was published (Siegel TN, Hekstra DR, Kemp LE, Figueiredo LM, Lowell JE, Fenyo D, Wang X, Dewell S, Cross GA. Four histone variants mark the boundaries of polycistronic transcription units in Trypanosoma brucei. Genes Dev. 2009 May 1;23(9):1063-76). Later, the viewer was also used to investigate replication arrest at highly expressed genes in Sacharomyces cerevisiae.