ANALYSIS OF MASS-SPECTRA OF CROSS-LINKED PEPTIDES

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 我们有一个正在进行的项目，使用交联剂来探测在体外和体内与目标蛋白相互作用的蛋白质，并最终确定这些蛋白质之间的相互作用部位。我们已经编写了软件，帮助我们通过对各种酶消化后的交联蛋白进行质谱分析来推断这一信息。目前，我们正在测试甲醛和戊二醛的使用情况。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We have an ongoing program to use crosslinking to probe for proteins that interact with a target protein both in vitro and in vivo and ultimately to determine the sites of interaction between these proteins. We have written software that will help us to deduce this information by mass spectrometric analysis of the crosslinked proteins after digestion by various proteases. Currently we are testing the use of formaldehyde and glutaraldehyde for this purpose.