PROMOTER-BASED FUNCTIONAL MAPPING OF AMYGDALA MICROCIRCUITS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该项目使用细胞类型的特定启动子来驱动荧光记者，以帮助对啮齿动物和灵长类杏仁核器官型切片培养中的特定神经元亚群进行光引导电生理记录。 在本报告所述期间，我们使用带有绿色荧光蛋白(GFP)的CCK启动子，继续研究了含有CCK的中间神经元的生理和形态特性。然后，这种慢病毒构建物被用来在体内转染神经元，只有那些正常表达CCK的神经元才共同表达GFP。我们用这种方法研究了大鼠杏仁基底外侧核CCK中间神经元的生理特性，这项研究的手稿已被《神经生理学》杂志接受。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project uses cell-type specific promoters to drive fluorescent reporters to aid in optically guided electrophysiological recording from specific subpopulations of neurons within organotypic slice cultures of the rodent and primate amygdala. During the reporting period, we continued to investigate the physiological and morphological properties of cholecystokinin (CCK) containing interneurons using the CCK promoter tagged with a green fluorescent protein (GFP). This lentiviral construct was then used to transfect neurons in vivo, only those neurons that normally express CCK co-expressed GFP. We have used this approach to examine the physiological properties of CCK interneurons in the rat basolateral amygdala, and a manuscript on this study has been accepted by the Journal of Neurophysiology.