EARLY DETECTION OF POLYCYSTIC OVARIAN SYNDROME

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 目的：研制首个多囊卵巢综合征（PCOS）的诊断试验。 多囊卵巢综合征（PCOS）的特点是由于无排卵、雄激素和其他激素分泌异常以及胰岛素抵抗而导致不孕。多囊卵巢综合征是最常见的女性内分泌疾病，影响4%至7%的育龄妇女，该综合征占所有无排卵的75%。简而言之，多囊卵巢综合征对妇女的生殖健康有着惊人的不利生理、心理和经济后果。 我们正在开发一种新的“代谢分析”方法，以检测和统计模型中的一个子集的分子在体内的总池的代谢物，已被证明是可靠的PCOS的早期指标的变化。 该方法将来自测量妇女以及威斯康星州国家灵长类动物研究中心的多囊卵巢综合征恒河猴的生物标志物。在过去的二十年里，这些动物已经被很好地描述和研究。这个想法是使用非人灵长类PCOS模型与人类样本平行，以开发一种新的诊断测试。有了这个，我们将开始分析患者样本，以开发人类PCOS的肖像。我们的方法可以用于开发各种其他疾病的类似测试。 我们将使用UW独有的技术和资源组合麦迪逊。我们将使用稳定同位素，NMR和质谱，以及先进的数学计算，所有这些都围绕着临床和医学科学，具有广泛的临床应用。 这项研究使用WNPRC动物服务。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To develop the first diagnostic test for PCOS. Polycystic ovary syndrome (PCOS) is marked by infertility due to anovulation, abnormal secretion of androgens and other hormones, and insulin resistance. PCOS is the most common female endocrine disorder, affecting 4 to 7 percent of women in their reproductive years, and the syndrome accounts for 75 percent of all anovulations. In short, PCOS has staggering adverse physiological, psychological and financial consequences for women's reproductive health. We are developing a novel "metabolic analysis" method to detect and statistically model changes in a subset of molecules within the body's total pool of metabolites that have proven to be reliable early indicators of PCOS. The method will derive from measuring biomarkers in women as well as in rhesus monkeys with PCOS at the Wisconsin National Primate Research Center. These animals have been well characterized and studied during the past two decades. The idea is to use a nonhuman primate model of PCOS in parallel with human samples to develop a novel diagnostic test. With this, we will begin to analyze patient samples to develop the portrait of PCOS in humans. Our approach could be used to develop similar tests for a variety of other diseases. We will use a combination of technologies and resources unique to UWMadison. We will use stable isotopes, NMR and mass spectroscopy, and advanced mathematical computation, all of which are centered around clinical and medical sciences with broad clinical applications. This research uses WNPRC Animal Services.