THE CART RECEPTOR

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 为了充分利用和应用我们对CART肽的知识，CART受体的鉴定和克隆是必不可少的（可能存在几种受体）。 筛选与CART肽相关的药物需要与受体一起工作并表达受体的能力。 虽然已经有几篇关于受体的出版物，但我们已经开始了深入的研究，目的是改进受体的测定和克隆。 CART受体结合试验已经标准化，并且已经测量了几种细胞系中的特异性结合水平。 此外，通过使用相对罕见的技术方法，我们已经能够首次在大鼠大脑中鉴定特异性CART受体结合。 寻找CART肽受体的另一种方法是研究已知的孤儿受体（孤儿受体是已知的受体，但其激活底物是未知的）。 正在测试具有特定孤儿受体缺失的敲除小鼠对CART肽的应答。 我们继续在鉴定CART肽受体方面取得重大进展。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To fully utilize and apply our knowledge of CART peptides, identification and cloning of the CART receptor is essential (it is possible that there are several receptors). Screening for medications related to CART peptides requires an ability to work with and express the receptor. While there have been several publications on the receptor, we have begun an intensive study with the goal of improving assays and a cloning of the receptor. The CART receptor binding assay has been standardized, and levels of specific binding in several cell lines have been measured. Also, by using a relatively rare technical approach, we have been able to identify specific CART receptor binding in rat brain for the first time. Another approach to finding the CART peptide receptor has been to study known orphan receptors (an orphan is a receptor that is known, but whose activating substrate is unknown). Knockout mice with deletions of specific orphan receptors are being tested for responses to CART peptides. We continue to make significant progress identifying the CART peptide receptor(s).