Shared Resources - Radiation and Free Radical Research Core

项目总结(见说明)： 辐射和自由基研究核心专注于为HCCC提供最先进的技术 研究人员研究氧化应激和氧化还原生物学的作用，因为它们与癌症生物学和 癌症治疗。越来越多的证据表明，氧化应激和氧化还原生物学是 癌症生物学包括启动、促进和发展为恶性肿瘤的过程以及 预防和治疗癌症。辐射和自由基研究核心(RFRRC)是 建立的目的是提供自由基和辐射生物学专业知识、试剂、技术和 HCCC研究人员进行基础、临床前和临床研究的分析。核心提供的三项基本服务是： 1)电离辐射服务和磷成像以及细胞周期分析工具，对了解细胞对放化疗的反应至关重要。 2)电子顺磁共振波谱和其他检测方法，用于测量自由基、单线态氧、一氧化氮和相关氧化剂和氧化损伤产物的阵列。 3)抗氧化酶服务，提供修改和测量负责促氧化剂形成、活性氧代谢的分子和氧化还原生物的介体的技术，包括：抗氧化蛋白质、小分子细胞硫醇和还原剂，以及氧化还原介导的控制生长、分化和细胞损伤的信号和基因表达途径。 流程。 RFRRC的独特之处在于它能够为HCCC成员提供方便地获取此类知识、试剂和资源的能力。RFRRC的专业知识基于自由基癌症生物学计划，但RFRRC在2005-2009年间有80多名HCCC成员使用其设施，代表了所有6个HCCC计划。在这一支持期间，RFFRC的服务为研究活动提供了便利 对135份同行评议的出版物作出了重大贡献。

PROJECT SUMMARY (See instructions): The Radiation and Free Radical Research Core focuses on providing state of the art technologies to HCCC investigators studying the role of oxidative stress and redox biology as they relate to cancer biology and cancer therapy. There is growing evidence that oxidative stress and redox biology are critical determinants of cancer biology including the processes of initiation, promotion, and progression to malignancy as well as the prevention and treatment of cancer. The Radiation and Free Radical Research Core (RFRRC) was established to provide easy access to free radical and radiation biology expertise, reagents, technologies, and analysis for HCCC investigators doing basic, pre-clinical, and clinical research. The three basic services provided by the core are: 1) Ionizing radiation services and phosphorimaging as well as cell cycle analytical tools critical to understanding cellular responses to radio-chemo-therapy. 2) Electron paramagnetic resonance spectroscopy and other detection methodologies for measuring free radicals, singlet oxygen, nitric oxide and the array of related oxidants and oxidative damage products. 3) Antioxidant enzyme services to provide easy access to technologies for modifying and measuring molecules responsible for pro-oxidant formation, metabolism of reactive oxygen species, and mediators of redox biology including: anti-oxidant proteins, small molecular weight cellular thiols and reductants, as well as redox mediated signaling and gene expression pathways governing growth, differentiation, and cell injury processes. The RFRRC is unique in its ability to provide HCCC members easy access to such knowledge, reagents and resources. The expertise for the RFRRC is based in the Free Radical Cancer Biology Program, but the RFRRC had more than 80 HCCC members use its facilities from 2005-2009, representing all 6 HCCC programs. During this period of support the research activities facilitated by the services in the RFFRC significantly contributed to 135 peer reviewed publications.