MicroArray

项目总结(见说明)： 微阵列共享资源允许CSHL癌症中心成员利用强大的基因组学工具和技术。共享资源提供全方位的微阵列分析，主要关注Affymetrix和Agilent平台。该设施使用的微阵列应用程序提供RNA图谱、拷贝数变异的基因组分析以及对DNA/蛋白质相互作用的洞察 染色质免疫沉淀法修饰染色质。微阵列服务的使用促进和加强了与生物信息学和DNA测序共享资源的多学科互动。微阵列提供了不同细胞类型或不同条件下基因表达变化的低分辨率、全基因组视图，作为途径分析和 癌症基因发现实验中关键变异区域的识别。在许多情况下，微阵列实验可以作为高通量测序的前奏，并有助于集中测序工作。

PROJECT SUMMARY (See instructions): The Microarray Shared Resource allows the CSHL Cancer Center members to take advantage of powerful genomics tools and techniques. The Shared Resource provides full service microarray analysis, primarily focusing on the Affymetrix and Agilent platforms. The microarray applications used by the facility provide RNA profiling, genome analysis of copy number variation and insights into DNA/protein interactions and chromatin modifications by means of chromatin immunoprecipitation. The use of Microarray services has promoted and strengthened multidisciplinary interactions with the Bioinformatics and DNA Sequencing Shared Resources. Microarrays provide a low resolution, genome-wide view of changes in gene expression in different cell types or under different conditions, which serve as a starting point for pathway analysis and the identification of key regions of variation in cancer gene discovery experiments. In many cases, microarray experiments can be a prelude to high throughput sequencing and can help focus the sequencing efforts.