Genomics

项目总结(见说明)： 基因组学设施(GF)是通过合并以前批准的CCSG核心(研究细胞遗传学和激光捕获显微切割、DNA微阵列和癌症预防生物标记和基因分型设施)而创建的，目的是提高成本效益和效率，巩固空间，增加工作人员之间的智力和技术交流，并提供对实验室重要的交叉培训 稳定性。此外，实时聚合酶链式反应服务，以前是生物化学和生物技术设施的一部分，被整合到合并后的GF中，以避免冗余。这次合并是由设施母公司监督委员会(FPOC)建议的。FPOC是一个机构委员会，由一名教席和中高级教员组成。FPOC的任务是确定CCSG资助的共享资源的优先顺序。GF由四个组成部分组成：细胞遗传学和染色体微阵列分析， 激光捕获显微切割(LCM)、DNA微阵列、基因分型和实时聚合酶链式反应。细胞遗传学和染色体微阵列分析服务执行经典细胞遗传学和分子细胞遗传学，以及用于检测基因组失衡的高分辨率DNA拷贝数分析，包括染色体缺失和扩增。LCM服务提供所需的工具和专业知识 用于从异质组织样本中分离纯细胞群体。从捕获的细胞中纯化的DNA、RNA或蛋白质可用于广泛的下游应用，如基因表达分析、DNA测序和蛋白质组分析。DNA微阵列服务为研究人员提供癌症研究的基因表达分析解决方案。该单位服务包括微阵列 实验设计、RNA样本标记、杂交和扫描。基因分型和实时聚合酶链式反应服务为全基因组和靶向DNA和RNA分析以及定量实时聚合酶链式反应服务提供各种平台和专业知识。2009年，36名经同行审查、资助的调查人员使用了GF。合并以前不同的核心提供了运营和经济上的优势。 该机构为合并后的业务提供了毗连的实验室空间，以促进交叉培训、设备共享和工作流程效率等协同增效作用。此外，合并还带来了真正的成本节约。统一运营使以前作为独立核心提供这些服务的FTE总数减少了1.7。

PROJECT SUMMARY (See instructions): The Genomics Facility (GF) was created by merging previously approved CCSG cores (Research Cytogenetics and Laser Capture Microdissection, DNA Microarray, and Cancer Prevention Biomarker and Genotyping Facilities) in order to increase cost-effectiveness and efficiency, consolidate space, increase intellectual and technical communication among staff, and provide cross-training important for laboratory stability. In addition, the Real-Time PCR service, previously part of the Biochemistry & Biotechnology Facility, was integrated into the merged GF to avoid redundancy. This merger was recommended by a Facility Parent Oversight Committee (FPOC). The FPOC is an institutional committee comprised of a Faculty Chair and mid to senior level faculty. The FPOC mandate is to prioritize CCSG funded shared resources. The GF consists of four component services: Cytogenetics & Chromosome Microarray Analysis, Laser Capture Microdissection (LCM), DNA Microarray, Genotyping and Real-time PCR. The Cytogenetics and Chromosome Microarray Analysis services performs classical cytogenetics and molecular cytogenetics, as well as high-resolution DNA copy number analysis for the detection of genomic imbalances, including chromosomal deletions and amplifications. The LCM service provides instrumentation and expertise needed for isolating pure cell populations from heterogeneous tissue specimens. The purified DNA, RNA or protein from captured cells can be used for a wide range of downstream applications such as gene expression analysis, DNA sequencing, and proteomic assays. The DNA Microarray service provides investigators with gene expression analysis solutions for cancer research. The services of this unit include microarray experimental design, RNA sample labeling, hybridization and scanning. The Genotyping and Real-time PCR service provides various platforms and expertise for genome-wide and targeted DNA and RNA analyses as well as quantitative real-time PCR services. Thirty-six peer-reviewed, funded investigators utilized the GF in CY2009. Merging the previously distinct cores has provided both operational and economic advantages. The institution has provided contiguous laboratory space for this merged operation to promote synergies such as cross training, sharing of equipment and efficiency of workflow. In addition, the merger has produced real cost savings. Unifying the operation has reduced by 1.7 the total FTE's dedicated to providing these services previously as separate cores.