Core Grant for Vision Research

描述（由申请人提供）：这个P30核心补助金的视觉研究申请从威斯康星州视觉研究人员的大学提出了三个模块;基因传递/定量分子生物学;病理学和成像;和动物模型和眼器官培养。有效地将基因递送到细胞的能力是治疗眼部疾病的一种强大的新治疗方法，并且是一种重要的研究工具。基因传递模块将构建载体，制备这些材料的高滴度储备供研究人员使用，并协助研究人员使用基因传递方法。定量方法在分子生物学中的应用越来越广泛。特别是通过RT-PCR定量基因表达和使用阵列技术分析基因表达变化。基因递送/定量分子生物学模块将为这些领域提供专门的技术专长，并将提高目前使用这些技术的研究人员的能力，将其添加到他们的研究计划中。随着对转化研究的重视，病理学分析和先进的成像方法变得越来越重要。病理学和成像模块将提供先进的组织学、显微镜和图像分析，以及组织切片和培养细胞的形态测定。新疗法的开发和提高我们对视觉系统功能的理解依赖于将体外工作转移到动物模型上。动物模型和眼器官培养模块将帮助研究人员使用动物模型（灵长类动物，猫，兔和啮齿动物），实验性青光眼诱导，成像和眼生理学。这种专业知识将有助于核心用户的努力，以协助他们的感染因子，药物，操作和载体/结构对啮齿动物，猫，兔和灵长类动物的眼功能的影响。在上一个资助期内，共有28名符合资格的非政府机构赠款获得资助。截至提交日期，188篇同行评审论文涉及使用核心。

DESCRIPTION (provided by applicant): This P30 Core Grant for Vision Research application from the University of Wisconsin Vision Researchers proposes three modules; Gene Delivery/Quantitative Molecular Biology; Pathology and Imaging; and Animal Model and Eye Organ Culture. The ability to efficiently deliver genes to cells is a powerful new therapeutic approach in treating ocular disease and is an essential research tool. The Gene delivery module will construct vectors, prepare high titer stocks of these materials for use by researchers, and assist investigators with gene delivery methods. The use of quantitative methods in molecular biology is growing. In particular, the quantitation of gene expression by RT-PCR and the analysis of gene expression changes using array technology. The Gene Delivery/Quantitative Molecular Biology Module will provide dedicated technical expertise to these areas and will enhance the abilities of those researchers currently using these technologies to add them to their research programs. Pathology analysis and sophisticated imaging methods have become increasingly important, as the emphasis on translational research has grown. The Pathology and Imaging Module will provide advanced histology, microscopy and image analysis, and morphometry of tissue sections and cultured cells. The development of new therapies and improving our understanding of visual system function relies on transferring work done in vitro to animal models. The Animal Model and Eye Organ Culture Module will assist investigators in using animal models (primate, cat, rabbit and rodent), experimental glaucoma induction, imaging and ocular physiology. This expertise will aid the efforts of core users in assisting the effects of their infectious agents, drugs, manipulations, and vectors/constructs on ocular function in rodents, cats, rabbits and primates. During the previous funding period a total of 28 qualifying NEI grants were assisted. As of the submission date, 188 peer reviewed papers have involved use of the core.