Rocky Mountain Neurological Disorders Core

斑马鱼鱼芯 C3.1基本原理。Core C为RMNDC调查人员保留了野生型、转基因和突变斑马鱼品系。 斑马鱼胚胎独特的发育和光学成像特性使其成为解决RMNDC研究项目核心问题的理想模型。UCD NINDS研究人员是国际公认的神经生理学、神经发育和光学成像领域的专家。 在之前的获奖期内。核心C消除了对使用模型系统感兴趣的RMNDC调查人员的技术障碍(例如，Betz、Caldwell、Clouthier、Niswander、Restrepo、Taylor、Williams) 但没有维护线路的经验或手段。 我们为核心C提出了三个具体目标，使RMNDC成员能够利用斑马鱼系统进行神经发育和行为研究。除了目前的13名Core C用户外，该设施还将允许因维护和饲养斑马鱼相关的实用限制而未考虑使用斑马鱼模型的调查人员访问该模型。特别是，已经有了 对补充啮齿动物研究的兴趣日益增长(例如核心B)与斑马鱼(例如，阿佩尔、阿廷格、巴洛、Clouthier、Macklin、Niswander、Restrepo、Williams)进行比较，反之亦然。核心也将是更大的科学界的资源，因为转基因品系将在RMNDC网站上描述，并在第一次发表时提供给斑马鱼国际资源中心(Zirc)。

Zebrafish Core C3.1 Rationale. Core C maintains wild type, transgenic and mutant zebrafish strains for RMNDC investigators. The unique developmental and optical imaging properties of the zebrafish embryo makes it an ideal model to address questions at the heart of the research programs RMNDC investigators. UCD NINDS researchers are internationally recognized and expert in the fields of neurophysiology, neurodevelopment and optical imaging. During the previous award period. Core C removed technical barriers for RMNDC investigators who were interested in using the model system (e.g., Betz, Caldwell, Clouthier, Niswander, Restrepo, Taylor, Williams) but did not have experience or means for maintaining lines. We propose three Specific Aims for Core C that will allow RMNDC members to take advantage of the zebrafish system for neurodevelopmental and behavioral studies. In addition to the 13 current Core C users, the facility will allow access to the zebrafish model to investigators who have not considered its use because of the pragmatic limitations associated with maintaining and breeding zebrafish. In particular, there has been growing interest in complementing rodent studies (e.g.. Core B) with ones done in zebrafish (e.g., Appel, Artinger, Barlow, Clouthier, Macklin, Niswander, Restrepo, Williams), and vice versa. The core will also be a resource for the larger scientific community because transgenic lines will be described on the RMNDC website and made available to the Zebrafish International Resource Center (ZIRC) by the time of the first publication.