Cellular Imaging Core

E.理由 该核心的一个关键目标是为CN和GWU的研究人员提供最先进的成像技术，以在IDDR这一领域对细胞和动物模型进行研究。此外，细胞成像和分析中心通过工作人员和附属用户的专业知识协助研究人员对各种儿童疾病进行研究。提供的服务包括：1）样品制备，通过光学显微镜和EM检查细胞和组织（见项目 * 1-6）; 2）对每个项目获得的数据进行定量分析; 3）获得细胞和组织样品，进行基因组学/蛋白质组学分析（见项目#6）。对于这些服务，我们利用核心部门的专职工作人员，并通过 具有特定专门知识的用户的共同参与（见E.2，3节）。此外，我们还与其他核心合作，包括基因组学和蛋白质组学以及生物统计学和信息学核心。使用最近获得的激光捕获显微镜（见第E.6节）增强了我们从动物或患者材料中靶向提取病变细胞和组织的能力，并在基因组学和蛋白质组学核心的帮助下进一步增强了对这些材料的分析。此外，为建立神经细胞培养物和细胞系提供的服务对于这些纯化的细胞的后续分析是有价值的。 通过基因组学和蛋白质组学分析，对细胞群的分子谱进行定量分析。在未来几年，我们将增加我们的核心提供的支持，进行实时成像和成像数据的定量分析。为此，我们聘请了一位在这两个方面具有丰富和出色经验的新总监，并聘请了一位具有定量图像分析背景和多年经验的新核心经理。此外，我们已经开始与生物统计学和信息学核心密切合作，使用户能够适当地设计和执行适当的数据统计分析。我们还与全国其他IDDRC密切合作，其中一个例子是科尔宾博士和亨茨曼博士与约翰霍普金斯医学院肯尼迪克里格研究所的IDDRC合作开展的工作。他们与Walter Kaufmann博士（脆性X综合征领域的专家临床医生和科学家）的合作利用了细胞成像核心，最近发表了关于脆性X综合征小鼠模型中抑制性突触缺陷的出版物（Olmos-Serano et al.，2010年）。

E. Justification A key goal of this Core is to provide access to state-of-the-art imaging technologies to researchers at CN and GWU to perform studies on cells and animal models in this area of IDDR. In addition, the Cellular Imaging and Analysis Core assist investigators in their studies on a variety of children's diseases through the expertise of the staff and the affiliated users. The services offered include assistance in 1) preparation of samples and examination of the cells and tissues by light microscopy and EM (see projects* 1-6), 2) quantitative analysis of the data obtained for each project and 3) obtaining cells and tissue samples to carry out genomics/proteomics analysis (see project#6). For these services we make use of dedicated staff in the Core and also through the communal participation of the users with specific expertise (see section E.2, 3). Additionally, we work together with other Cores including, the Genomics and Proteomics and the Biostatistics and Informatics Cores. Use of the recently acquired laser capture microscope (see section E.6.) has enhanced our ability for targeted extraction of diseased cells and tissues from animal or patient materials further enhancing the analysis of these materials with the help of the Genomics and Proteomics Core. Additionally the services provided for establishing neural cell cultures and cell lines have been valuable for subsequent analysis of these purified populations of cells for quantitative analysis of their molecular profiles through genomic and proteomics analysis. In the coming years, we will increase the support our Core provides in carrying out live imaging and quantitative analysis of imaging data. To this end, we have a new Director with extensive and outstanding experience in both these aspects and have hired a new Core Manager who has background and several years of experience in performing quantitative image analysis. In addition, we have started working closely with the Biostatistics and Informatics Core to enable the users to appropriately design and carry out suitable statistical analysis of their data. We are also working in close collaboration with other IDDRCs nationwide - one example of which is the work that Drs. Corbin and Huntsman's have been performing in collaboration with the IDDRC at the Kennedy Krieger Institute at John's Hopkins Medical School. Their work with Dr. Walter Kaufmann (an expert clinician and scientist in the area of Fragile X Syndrome) makes use of the Cellular Imaging Core and has recently resulted in a publication on inhibitory synaptic defects in a mouse model of Fragile X Syndrome (Olmos-Serano et al., 2010).